FastQCFastQC Report
Wed 2 Jan 2019
HC-A-2_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-A-2_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959804
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT790760.5664549444963555No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA691510.49535795774783087No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT516580.37004817546148927No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA505540.3621397549707718No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG386250.27668726580974917No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG352680.2526396502415077No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG349350.2502542299304489No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG338570.24253205847302728No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG316180.22649315133650874No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG303490.21740276582679816No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC293130.20998145819239294No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC284040.2034699054513946No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG282660.20248135289005492No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC280930.20124208047620154No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA268120.19206573387420053No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT266700.1910485276154307No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT262730.18820464814548973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261000.18696537573163635No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT257720.18461577254236522No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA255260.18285357015041184No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT219830.15747355765166904No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT212820.15245199717703772No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC211960.1518359426822898No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA207410.14857658459961187No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC200120.14335444824296958No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG199140.14265243265593128No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC199140.14265243265593128No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG183880.13172104708633445No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA180760.12948605868678384No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG177890.12743015589617163No Hit
GCGGATATGGGTACGGCCTGGCACGAAAGTCACAATGTCTCCCTCGGATT175820.1259473270541621No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT172470.1235475798943882No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG168150.12045298057193354No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC165280.11839707778132129No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG164320.11770938904299803No Hit
ATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCA161290.11553887146266524No Hit
GGTGAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAG156860.1123654744722777No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT156390.1120287935274736No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCAC154990.11102591411741884No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA154280.11051731098803393No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT150210.10760179727451762No Hit
CTTGTTTGCTATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTAC146920.10524503066088894No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT145270.10406306564189584No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA143450.10275932240882465No Hit
CTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCGC140680.10077505386178774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC158950.069.3165747
TACGCTA160050.068.485469
ATATACG163200.067.5107356
AATATAC172250.064.342465
ATACGCT170650.064.183928
CTTTAAT173400.063.8200721
TAATATA175650.062.9741024
GTTAATA200650.062.783731
TCATATG74150.061.3116042
GCGCCAT132400.061.0567551
GTCATAT75700.060.296391
CATATGC75700.059.847073
TTTAATA188650.058.8059042
TATTCCC227350.055.093226
TAATATT228600.055.0785263
ATTCCCG235500.053.2795077
ATATTCC238100.052.9711345
GCCATCC154500.052.5939563
GCTCGGG56800.051.045925
CATCCAT164750.049.012765
Produced by FastQC (version 0.11.5)