FastQCFastQC Report
Wed 2 Jan 2019
HC-A-2_S5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC-A-2_S5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959804
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1254450.8986157685308477TruSeq Adapter, Index 2 (97% over 37bp)
GTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGT832530.5963765680377747No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA790610.5663474931309924No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTACTTGT543120.3890599037063844No Hit
GCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACA434710.3114012202463588No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAG388170.2780626432863957No Hit
GGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTG363950.2607128294924485No Hit
GCCAGTTCTAAGTTGGCTGTTAGTCGCCGGACGAAGCCGACGGCCTCGGG357930.256400448029213No Hit
GCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGG344320.2466510274786093No Hit
CTCGCTTCGACCGACACCCGAAATGAGCAACGGGTGCGTGTGGTCGTCTG332750.23836294549694248No Hit
GAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG305910.21913631452132135No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC299820.21477378908758316No Hit
CCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCAT297310.21297576957384218No Hit
CTAACATGTGCGCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGA296310.21225942713808876No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCAGAGCGGATCGCCCCCGGCCGC288230.20647138025720133No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACATACTC280960.20126357074927415No Hit
GCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG266440.1908622785821348No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA264540.18950122795420338No Hit
GTTAGTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCT263030.18841955087621573No Hit
GCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT262710.18819032129677465No Hit
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG257100.1841716402321981No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT247560.1773377333951107No Hit
GCAAGTCATTGGGTGTTACGAAACCTAAAGGCGTAATGAAAGTGAAGGCA225220.16133464338037984No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTACACC224880.16109108695222368No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACATACTCTTGCACAGTGAAACC222450.15935037483334294No Hit
GTTTTACCCTACTGATGACTGCAGTCGTTGCAACGGTAATCCTGCTCAGT216170.1548517443368116No Hit
ATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGCCTG210420.15073277533122958No Hit
CCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGTCGTGTGCGGTAACG202870.14532438994129146No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC199420.14285300853794222No Hit
AAAGAAACTAACTAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG197560.1415206116074409No Hit
GTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTCACGGTA180840.12954336608164413No Hit
GTTTATGGTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACT178760.12805337381527707No Hit
TCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGTGGTGCG178000.1275089535641045No Hit
ATTACCTCGTGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCA176650.1265418912758374No Hit
ATCGGGTTAATATTCCCGAACCTGGCAACGGAGTTGGCTCCTCGCGGGGT174660.12511636982868815No Hit
GCGGATATGGGTACGGCCTGGCACGAAAGTCACAATGTCTCCCTCGGATT174610.12508055270690047No Hit
CTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC160240.11478671190512417No Hit
GTACGCTCTTGCTCCGCCCCTTCGACGCAGTGCGAACGGGACGGGCCGGT158930.11384830331428722No Hit
GAAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTTCGCCT155240.11120499972635718No Hit
CTTGTTTGCTATCGGACTCGGGGAAGTATTTAGCCTTAGATGGAGTTTAC154510.1106820697482572No Hit
GGTGAACAAGCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAG154490.11066774289954213No Hit
TGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTACTCTACGCGAGGTTT154150.110424186471386No Hit
GGGAATTCCTCGTTCATGGGGAGAATTACACACCCCAATCCCTAGCACGA152390.10916342378446No Hit
GTAGATCTGGGCGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCA151770.1087192914742929No Hit
CGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCT149060.1067780034734012No Hit
GAAAGGCTTAGTCTCAATAGATCGCAGTGTGGTGGCTGCTCTACTAGGTA145830.10446421740591774No Hit
CGAGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTT144810.10373354812144926No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAACCTACTAAA140270.10048135346312885No Hit
GTTATTTTTCGTCACTACCTCCCCGTGCCAGGAGTGGGTAATTTGCGCGC139680.10005871142603434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC169900.073.096587
TACGCTA170000.073.010949
ATATACG173000.071.923566
TAATATA173900.071.1760564
AATATAC176950.070.19515
ATACGCT178950.069.44048
CTTTAAT181200.069.11091
TTTAATA179650.068.8167342
GCTCGGG68700.064.3832865
GTTAATA197550.063.1340751
TCATATG72650.059.3852042
GTCATAT74650.059.0590131
CATATGC74850.057.3493273
TAATATT219250.056.850553
TATTCCC229350.054.1574976
GCGCCAT126550.054.0333561
ATATTCC237700.052.560095
ATTCCCG237000.052.4318967
TCGGGGG86850.051.1709827
TTAATAT367350.048.7348333
Produced by FastQC (version 0.11.5)