FastQCFastQC Report
Wed 2 Jan 2019
Edm-R_S30_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEdm-R_S30_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238647
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAACAATCAATTTAACTCGACAACAATGACAATGAGATTATTATAAT164666.899730564390083No Hit
GTTGATGCTGCTGTTGTTGTTATTGTTGTTGTTGTCTGTGTTTATCGAAT95854.0163924122239125No Hit
AAACAGTCGCCTGGGTCATTTCTCTGCGGCCTCCAGAGGCTAAGTACGCG44581.86803102490289No Hit
CGACTGCGTGGTGTTTCGGGGATGGTGGTGATTGACTCGAGGTTGGTGGA40271.687429550759071No Hit
AGGCTGAACTCGCCTATTATTGTCGGATGAGGTGATGTGGAGGAGTTCTT39141.6400792802758888No Hit
AAACTAACACAACAGCGACGTCAGACGCCTTCTGGGGCCCATCGTATTGG35961.5068280766152518No Hit
GGGCGCGTTTCTGGCGGTGCCACAACTGCCACAGCAACCAGCCGAGCAGC33861.418831998726152No Hit
GATGGGCATCGTAGGTTGAGGAGGATGCTTTCGAGCAGCTCCGGAATAGC29111.2197932511198548No Hit
CTTCTCGATCAACCCAGATGAAGCAATTGCATATGGCGCAGCAGTTCAAG27941.1707668648673564No Hit
GTGGTCCCGGATCTTAGGCCGCTCGCAGATCACGACCGCGTCCACGTTCG25131.0530197320728942No Hit
CATAAATCGCAAAGAGCTTTTCCAATGCATGTCAAAAGCACCATAGTGAC18040.7559282119616002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17660.7400051121530965No Hit
TCTGGCTGCTGATCGGCCTGCTCCTGGTCTCATCGCTGCTCTCCGCCCTG17030.7136062887863664No Hit
ATAAAACTGCAGAACCAAAGGTTGGGCAGACTAAACCACAACCACAACCA15450.6473997158983771No Hit
GGGGAGTACGATCTATCCTCTATACGGCTGTGTGCCTCGGCGGCGGAGGC14680.6151344873390405No Hit
TCGCCCAGGGTGGTCGGCTTGAGCCCGAGGTCCAAAAAGCGGTCGTTGCG13830.5795170272410716No Hit
ACACGGTGGCCCATGACCTGGCCTTCCACACCCAGATCGCCGAGGCCACG13820.5790979982987425No Hit
CGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGC13220.5539562617589997No Hit
GGGTACTTTTCACCATTCCCTCACGGTACTGATCCGCTATCGGTCAACAG12310.5158246280070565No Hit
TGACCAGCAATGGGTTTACATCGAGTTCCAGCAACTGCCCGGCGTTGGCG11920.4994824992562237No Hit
GCGGCTCGCGGGTCTCTCCACCCTCCCCCTGCGTCCTCCTCCGCCCTCCT11340.4751788206011389No Hit
GATTTCTCCAGCCTTCAGGTGACGATCGGTGGTGGCATGGCGGTGCAGCG11300.4735027048318227No Hit
CATGCAGCACCTGTGTTCTGGCTCTCTTTCGAGCACTCCCAAATCTCTTC10760.4508751419460542No Hit
AGTTCTCTGGGCCGCGCTGGCCCTAGTGGGAGGCGCGATCAACAAGGCGG10610.4445897078111185No Hit
GGCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGATGCTGGACCG10420.4366281579068666No Hit
TCACGCGCGCCTTTCAGGACCTGTTCTTCGGTGATGAGATTGAGGCGGCC9740.4081341898284915No Hit
ACGACAACGGCGTGCTGACGCTGACCCTGCCGAAGAAGGCGGCGGTGGCC9730.4077151608861624No Hit
GAAGACCTACCAAGACAACCCAACCCAAGCCATCCATCAAACATGAAGCT9730.4077151608861624No Hit
GTTGTGCGCCGCCTTCTCGGGGCTGCTCTTGTACGCGCTGTACAGAAAGC9650.40436292934753004No Hit
ATTGTGCAGGATTCACAGCTCGACACATATGTGTGATGTGATGTGAGAAG9580.4014297267512267No Hit
GTTCCCGACCTCGTGCGGGGCAGCGGCGAGCTCGTCGATGCGGACCAGGA9510.39849652415492337No Hit
GGCGGGCATCGGAAGAAGAAAATCAAACGTCGCCCGCGTCCATTGTGGCT9450.3959823505009491No Hit
GCCGAGTCGCCCTTCACCGCCAGATACCCGGCCTGCCCACGCTCCAGCGG9390.3934681768469749No Hit
GGTCTGGCTCCTCCTCGAAAGGCACCGCGACGACCACCCTGCGCCGCGCA9360.39221109001998766No Hit
GCCATGTAGTTTCGATCCTTAAAACCGGAGCCGATGCCATTCACAATACA8950.37503090338449674No Hit
GTGCGGCTGGATTTCCGGCTGGTGGCGGCCACCAACAAGCCGCTGCGCGG8900.3729357586728515No Hit
AGAGCCCTGATTATTGTACCACGGGTATCCAGATATTAGCTTGCGGACGC8520.35701265886434774No Hit
GCGACCGGTAGCGCGGTAAACATCAGGCCGACGGCTTTGCGAAACAGCGA8490.35575557203736063No Hit
CACAGGATCCAGAAGCATCTTATGATATTAACGATAATGATTCCGATCCA8440.3536604273257154No Hit
GCGGGACGAGCGCGGCTTCACGCTTCCCGAGCTACTTACGACGATTGCCA8240.34527984847913445No Hit
AGCCGCGCTTGGTCAACGTCAGGTTGAGCAGCCGCGCGCCGCGTTCCGCG8200.34360373270981825No Hit
ATAGCAAATTGATGGTGAGCAATTTACAAGGAAATTTCAAAATCGCAATG7980.33438509597857924No Hit
CTTGCTGTAGTCGTTACTGTCGTTAGTCAGTAAATTATGTGAGTCTTATA7690.3222332566510369No Hit
GCTGCTTGCTTGCTTGCCTATTTAGCTGCTGCTGCTGTGGATGTCATTTC7560.3167858804007593No Hit
GCTCAACCCAGTCTGTATCGTAGCTAACGCGATAAGTACACCGCCTGGGG7300.30589112790020406No Hit
GTAGTAATAGTAATAATAGTGATGAGATTGATGCCAACGCATCGGTGGTG7300.30589112790020406No Hit
CTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGACAGC7140.2991866648229393No Hit
CGTAAACGATGCATACTAGATGTAGTTGGGCTCAACCCTGGCTGTGTCGA7090.2970915201112941No Hit
GCCCATGGTTGTGGGTGAGTGAGTGATCCTGTACTGTCGAGAAAAGCTTC6900.28912997020704223No Hit
AGATGACAGAAAATAGAAGACTGCGTGAATTTTGGCTACTTTTTTGAGTG6760.28326356501443556No Hit
GCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACA6610.27697813087949985No Hit
GAGGACGGCGAGGACAGCGTGCAGGCCAGCGGCCGACCGGCGCCGGTCAG6580.27572104405251274No Hit
TGAATGACTAATAGAGCAATTAACATGAACATTATTACTCACCTTTATTT6540.2740449282831965No Hit
CCGCGGCCGCGAACGACAGCACGACGAGCGGCCACATGTGCCACACGAGC6120.2564457127053765No Hit
CACCTTGCATGCACGAGCTTAATTTAAATTAAATTTTCATATCCTGTCAC6040.2530934811667442No Hit
GCCGTCGCCAAGTACGACGTCGACCGCTACATCCACGTCTCCTCGCACTC5850.24513193126249228No Hit
CGCTCGTTGCGGGACTTAACCCAACACCTCACGGCACGAGCTGACGACAG5720.2396845550122147No Hit
GAGCGATGATCAATTCTCTTTCGTGGCGGCAGTGATGGCGCTTGCTCCTC5550.2325610629926209No Hit
CAACCCTTGTCGATGATGGCGTGCTCGATGGACTTGATGCCCACGGCGTC5510.23088494722330474No Hit
TCTGGGTCGGGCGCGCTGGTACGACTGGTATTCGCTGGACGTGACGCGGG5460.2287898025116595No Hit
CGCGCGCCAGGTCGCCCAATTCATCGCGGCGTTGCAGCGCAGGTACGCTG5410.22669465780001422No Hit
GTGCGCTCGCTGCGGGCGGCGGGCAAACAGGTCCCGGTGCTGTTCCTGAC5380.2254375709730271No Hit
GCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCG5300.22208533943439474No Hit
CGTAGTAGCACGGCCCCGTAACCGGTGGCGACGACACGCGGAAATGCACC5110.21412378953014283No Hit
GCTCTTCCTCCACCATCTCTCGTTCACTCTGTTAAGATTAGGCACCATTA5080.2128667027031557No Hit
AAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATT5080.2128667027031557No Hit
CGAGGCAGAGGCCGCGGAGCTTACCCACGACATGGCTACGACGGCGGCCA4940.20700029751054908No Hit
GCAAACTGGAGGACATAGTCACGAACAACCGCGTCGCCTTCGAGGCGGAC4910.2057432106835619No Hit
GCGCGCCAGAGCTGCCAGGCGATGTAGAACAGGTAGAACGCACCCAGCAC4880.20448612385657478No Hit
GTCGGCAATTGAGCATATCTCTCGCACACACACACAAGCGCACACACACA4720.19778166077931003No Hit
GGCTTCTATCTTCATCTCCTGGTTCCCGATGCTGTTGCTGATTGGTGTCT4670.19568651606766482No Hit
GTTGACCACTTCCAGGTTGACCAGGTGGCGGGCCACGCCGAATTCGTCGG4660.19526748712533573No Hit
ACTACCAGAACCCGCTGAACGTGGTCGGCACGCTGCCGGTCTGGGAGGCC4580.1919152555867034No Hit
GCCACTCCATGTCTGCACCTGCCCTGCGCTGCCCGCCTATAACATGTCGT4450.18646787933642575No Hit
CCTCGCCGCTAGCGTCGCGCGCCTCCACGCCGATCAGACCTCGCTCCTGC4430.18562982145176768No Hit
TTTCTTCTCAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTA4360.18269661885546434No Hit
GATTGAGCACCGCATAGCGCTGCACCAGCTGGCCGCCGCCGGAGTGGCCG4290.17976341625916104No Hit
GAGGAATGGATCAATATCGAATCTGTCAGCTGCTTCCTTAAAAGACACAA4260.17850632943217387No Hit
GATGGCGGCCCCACCGAGTCTGGTGGCAACCCGCCATCCGGTGGTATTGC4180.17515409789354153No Hit
GGTATATAGCAGTTTCAGTTTCCTCCACCGCACCGCACCGCACTTCTCGG4060.17012575058559296No Hit
GTGGAAACTCAAGAGACAACGAAAAAGACAAATTTACCACCAGCATCATC4020.16844963481627676No Hit
GGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGA3970.16635449010463155No Hit
CACACAGAACGGCGTGCCGACCTCGTCCTGCCTGCGGTAGCGCCGCCCTA3840.1609071138543539No Hit
ACAGCGAACTGGTTTTGCAAATGGGGGAAATATGGACTTTAGATTTCGCA3750.1571358533733925No Hit
AGGACATCTACGAGCTGACCTATATCCGCAAGGACAACAGTCGCCTGTCC3730.1562977954887344No Hit
GGCACGCCGGAGGGCTATACCGAGATCGCCGTCTCCGGCCGCACCGTGAC3650.15294556395010203No Hit
GGCGAGAAGATCGACATCATCCCGTGGTCGCCCGACACCGTGACCTTCGT3650.15294556395010203No Hit
GGGGCATCCTTTCAGCCAGGTTCTCAAGGATTATCTGGCCTTCCGCTACA3630.15210750606544393No Hit
GTCCGAGGCGGCCGAGTAGGGAAGGCGGGAAGCCAGACGTGGACGACACC3620.1516884771231149No Hit
CCATACTGTTGTGTCTTGAAATTTGGCGTTGCAAGGGGAGCTTGCTTCTT3530.14791721664215346No Hit
CGGGGGTGTCCGAATGGGGAAACCTGGAATGTCCGGAGTAGTGTCCGGTG3520.14749818769982442No Hit
CCCGAGGTGGATTCCGGGGACGGGGAGTAGGCGCTGGACTTCAAGTCCGC3500.14666012981516635No Hit
ACACCTTCGACGCGCTGGCACGTCGCTTCCTGGCCCCGCCGGTGCGCGCC3480.14582207193050825No Hit
GTGCCGTACTGTAGTCCAACAGCCTCTAATCGCCTCCAGCGACGCCGCGC3450.14456498510352112No Hit
GGCGTACGTTCGGAGGCTCCGAAGATCACCGCTGTAGCGTTCTCTCTAGC3440.14414595616119205No Hit
TCAAAAGAAGCCTACTTTTACGACTCGCCTAAGTCGCATGTTCTCAAACA3430.14372692721886302No Hit
CACCTCACTTCACTTCACTTCGCCAGCCTCGTCCGCGAGAAAGTCAAAAT3290.13786052202625637No Hit
GCGCTCCACGTGCTCGACGTCGTGGAAGAACTCGTAGGGGAAGCCCAGCG3290.13786052202625637No Hit
GCCTGCCAGCGGCGCAGGTCGGCCACGTCGTAGAGGATGACCTGCACCAT3260.1366034351992692No Hit
GCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCC3170.1328321747183078No Hit
GTGAGGTTGGCGATGCCGCCGAGGTTGAGCGCTGCCTTCGGCTCGTCGCC3150.1319941168336497No Hit
ATCACTGTTAGACCCTCACATAATCTTGCCCTTTCCTATGTCTTTAGCTT3020.1265467405833721No Hit
GCGCCACCGAAGCTCTAAGAGCCCCGACAGCTAGTTATCATCGTTTACGG3010.12612771164104306No Hit
GCGGGCCTTGAGCAGGGTGCGCCAGTGCTCCACCTTGTGCTCGCCGGAGA3000.125708682698714No Hit
GATCCCGTCGCCTCGATGCGCCACGGCCGCCGCATCGCGAGCCACACGTC3000.125708682698714No Hit
CTCGACGCCTTCGACGAGGAGGGCATAAAGTTCGCCTTCGAGGTTCACCC2950.12361353798706876No Hit
GGGGCATGATGATTTGACGTCGTCCTCACCTTCCTCCGAGTTGACCCCGG2950.12361353798706876No Hit
GCGAGCGATCGCCTGGTCGAGACCGGCGCGCACTTTATAGAAGCCTTCCT2940.1231945090447397No Hit
GCGAGATCTTTCCCGCCTCGCGCAACGGACTGATGCCAGCCTTGAACAGG2940.1231945090447397No Hit
CGACGATGTCGGCGGCGACCTGGCCTTGCGCGCCCGCGGCGACGGTGATG2920.12235645116008163No Hit
CTTGGCTGGGAAAGCTGAGCCACTCTTTGGAAGTGAGGCTGAGCTGTCAG2920.12235645116008163No Hit
CGCCCACCGTCAGGCTGCGCCCGACCATGCCCAGGAAACCGTCGGCGCCG2900.12151839327542353No Hit
CCACTCGCCGGTAGTGAAAGCTCCATAGGAGGCGTTGTCGGCGATGGTGT2870.1202613064484364No Hit
GTAAGGAATGCTTATGAAGACCACTAACGCCCAGCGCAAAGTCAACATCA2850.1194232485637783No Hit
GCGTAGCATGCGGATTAATTCGATGCAACGCGTAGAACCTTACCTGGGTT2850.1194232485637783No Hit
GGTGCGCAGGGCCGTTCAAGAGTCCGGCGTGCGGGATGGGTTCTGCGTCA2850.1194232485637783No Hit
ATTTGATCGGCATGGTCGGCTGGGTGAACAGCGCCTTGCCATTTTTGAGT2830.11858519067912021No Hit
GCGGCACCGTCGCCAAAATAGGCCATGGCGACGCCACCGTCACCGCGATA2820.11816616173679116No Hit
GTTCATTGACAGAACGGTTTTGAAAAAAATATGAATAAACAGTGTTGCCT2790.11690907490980401No Hit
CGGATGTTGAGAATAATCAATATCTTGATTGCCTGGCTGGTGCGGGTACG2770.11607101702514594No Hit
CCTTCGGGGACTGGGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTC2680.1122997565441845No Hit
ATAGCTCGCGCAGCCTGCCGACGGCGGTGGAGAAAGCTCCCAGAAAGCCC2660.11146169865952642No Hit
GGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA2640.11062364077486832No Hit
GAGTGAGGCGAGAGAGAATTTAGAGAAGCCAGAGAAAAACGAGAGTGAGG2600.10894752500555213No Hit
GCCACACGCCACTAACTCTCAGGCTCATTCTTCAAAAGGCACGCCGTCAC2560.10727140923623595No Hit
TCAAGGTCGAAGAGCTAAACCTGGATGTCGAGGAGCTGAAAGCCCGGGTG2560.10727140923623595No Hit
GGGTAACCCCGGCACCGGAGAAGGGTCGGACGCGACCAGCGGGTTGGTTC2550.1068523802939069No Hit
GTCGATGTGGTCGAAGGAGTTTGTGCACAGACGAGAACGATTCTGCAGCA2530.1060143224092488No Hit
GTGTAGCCCTAGACGTAAGAGCCATGCGGACTTGACGTCATCCTCACCTT2510.10517626452459072No Hit
CCAGCTTGCATCAGTGAAGAAATGCGGCCACATGGGTGGCAAGGTACTGG2490.10433820663993262No Hit
GGTGCCAACGCTTGCAAGTCGTTCTCGTAGCTCTGACGCAATTTCGAGCT2480.10391917769760356No Hit
GCCGGTTGCAGGCGACGCCGAGGGTGAGGGCTCCGCGCTCTCTTGCGGCG2480.10391917769760356No Hit
GCGAAGCCCTAGGAGCATGCCGAGGGGCGGTTGCCTCGTAAAAAGCCGCA2450.10266209087061644No Hit
GCAGCTGGGCGCGGGTGAACAACTGGCAATTGTCGGCCCCAGTGGTTGCG2430.10182403298595834No Hit
GAAAAACCTTACCTACCCTTGACATGTCTGGAATCCTGAAGAGATTTGGG2420.10140500404362929No Hit
GTCAATTCTTTTGAGTTTCACCCTTGCGAGCGTACTCCTCAGGCGGCATA2410.10098597510130025No Hit
CGGGCCGCGCCAGCGGCGCGCCTTCGCCCGGGCCCAGCAGCAGCACGTCG2390.10014791721664215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA100.0070871343145.00252145
TACAATC202.1270625E-6145.00252145
CTGTAAG351.0913936E-11144.972122
TCCGAGA306.493792E-10144.972122
CTATCTT450.0144.941746
TTGTCTA151.2308099E-4144.941743
CCTCGTG950.0144.941749
AGTGCCT202.1315082E-6144.941748
ATATAGC306.493792E-10144.941744
GTGTCGC100.0070960238144.941749
CATCGGG100.0070960238144.941745
GTACGAT1700.0144.941746
GCACAAC19050.0144.74331
CTAACAC4300.0143.256384
TAACACA4400.0141.64765
GGCGCGT4100.0141.436222
AACAATC19700.0140.527285
TCCCGGA3100.0140.26625
ACAATCA19850.0139.465366
CGACTGC4900.0139.200781
Produced by FastQC (version 0.11.5)