FastQCFastQC Report
Wed 2 Jan 2019
Edm-R_S30_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEdm-R_S30_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238647
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGAATTTCATGTAAATTTATGACAAAACATTGACATTTTTCCTCCAT167587.022087015550165No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT162606.8134106022703TruSeq Adapter, Index 27 (97% over 39bp)
GTTTCATGCGTAGAATATTAATCAGAGCCTCCAGGATGCAGAAGCAGCAG97804.0981030559780764No Hit
CGACTGCGTGGTGTTTCGGGGATGGTGGTGATTGACTCGAGGTTGGTGGA45971.9262760478866277No Hit
GGAAGCTTTATGCAGAACCGATGGGGCCCGAAAAACTTCCAAGAAAGAAC45781.9183144979823756No Hit
AAACTAACACAACAGCGACGTCAGACGCCTTCTGGGGCCCATCGTATTGG40641.7029336216252455No Hit
ACCTTCCGCACGCCAAATCTCAACAACATTATCGACGCCAGCACCACCAC38881.6291845277753334No Hit
AGGCTGAACTCGCCTATTATTGTCGGATGAGGTGATGTGGAGGAGTTCTT37161.5571115496947374No Hit
TGTACAGCGCGCCGCTGGCCTTGGCGCTGATCCTCAGCATGCTGCTGGTG34471.444392764208224No Hit
CTGAAAGACTGCTGCCTGGCGCGAATTCTTCGCTTCGAAGTCGTCGTTCA30041.2587629427564562No Hit
ATTTTCGGTTCACCGCGTGGTGCTGCAGCGATGTCTGTAAGGCTGAATGT28921.211831701215603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27921.1699288069826983No Hit
CTGGAAGACATCGGCCATTATTTCCCCGACACCGACGAGCAATACCGCGA26101.093665539478812No Hit
GGGTGAACGGGGTCATGCGGGGTGCACCTCCTGGAAGTCGGCATCGAAGA23640.9905844196658664No Hit
CTTGTGTATGAATTTATTCGAATGTTTGAGTGTGTGTGTATGTATGTATG18140.7601185013848907No Hit
GCCGTTGAAGGCGGAGCGGATGAGGGTGGTGTCGGAACCGAGGCGGGTGA17450.7312055043641864No Hit
GAAATGTGTTCCGCCGCTGTTGGAGGGTTGCCCGAGTAGCACGGGGCCCG16970.7110921151323922No Hit
TTGGCCACATATCTTTACAAATCTGTATAACCTTTTTTCTAAACTCACAA16480.6905596969582689No Hit
GCCGAGTCGCCCTTCACCGCCAGATACCCGGCCTGCCCACGCTCCAGCGG15070.6314766160898733No Hit
CGAGACCCACAGGGTAAGAGACCTGGCAAAGCTAGTCGCAGAGCGAGCCG14510.6080109953194467No Hit
ATGGGAGCACTCAGGCGGCGCTGGTCTTTTGCGCCAGGCGCAGGCGTTCG14370.6021445901268401No Hit
GTGGACACAGTCTGTGAGCGCCTGCCCCGGTGGGTGGGAAGCAGGACTCG13160.5514420881050255No Hit
GAATCCTCGTGGAATTGCTCAAGGACAGTCGCAGCCTGTTGCTGCCGACC12620.5288145252192569No Hit
AGCCTTCGATGATCGGCTTGCCCGTGATCGCCTTCCAGCGCTCGGCCACG10990.4605128076196223No Hit
TATCCTTGTCGGAGCGTGACGGCGAATCCCGGTGCGGGCGGCGGGGAGCC10910.45716057608098987No Hit
TTTCTTCTCAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTA10740.45003708406139614No Hit
GTACTTTTCACCATTCCCTCACGGTACTGATCCGCTATCGGTCAACAGGT10680.44752291040742187No Hit
CGATTCCGATCTGCTTCGTGATCACGCTGGGCTTCATGCACTTCTTCGGC10240.4290856369449438No Hit
CTCCGACGCCGCCGCCGATGACCACTATCCGCTTGCCGCTCAAGGGCACT9910.41525768184808526No Hit
AACATTGCGCGCAGGTCTCTTACACCGTCTCGCCACTCATCTGCTTCTCC9840.41232447925178195No Hit
GTCATCAGCAGCACCCACGACCTGGCCGCCACGCCCGCGCAGGGCCAGGA9810.4110673924247948No Hit
GGGGAGTACGATCTATCCTCTATACGGCTGTGTGCCTCGGCGGCGGAGGC9730.4077151608861624No Hit
AATCATTCTGACATATTAACATCATTTTTCAGTCATCGCACGGTGCTCAT9680.40562001617451715No Hit
CTCGACCTCGCCCGGAACGCCGCCCGGACGGACGGCACGGTTAGTTTCGA9650.40436292934753004No Hit
CGAGGTCGACCACCGCGACCACTCCGACGCGGAACGCGCGCACCTGCGCG9600.4022677846358848No Hit
CCAGCCCCCACGCGCACTATCTGTGTTGGCGCGCAGGGGCGATGGAGCGC9600.4022677846358848No Hit
GTCGAATGCTGCGGTACCTGTGGATGCGGCGGCGGGGAGGGCTGATGCGG9420.39472526367396193No Hit
GAATAGTATAGTAGAGTGAAGTGAAATCATCGCGGTCGTAAAATGACCAA9270.3884398295390262No Hit
GGGCGGCTCGCCCAGCTCCTTCATCGTGGCCTCGCCCACCACCTTGCGCA8950.37503090338449674No Hit
CCTGCACGTGCTGGAGCATCTGGAGTCCTCGTCCGCGAGGCAGGCTTTAC8840.37042158501887723No Hit
GCAAGCTCGGGCTGGTGGTGCTGGCGTTGGTGGCCGTGGTGGCCATCGCC8690.3641361508839416No Hit
GATTCGGATTCAAGCCTAGAGCTTTCGCCTCAACCGCATCATTCACAGTT8580.35952683251832207No Hit
GGAGAGCCTGAGCAGATAGTAGTCAGGTCCACCATCCCCTTCCTCTGGCG8450.35407945626804443No Hit
GAGAAGTGAGAGGTAGAATTCATGAGAAATTGTTATTCATAAATTTTCAC8440.3536604273257154No Hit
ATACAATCACGGGCATGAATTCCGCAACACAGACCACCGCCGCCGAGGCC8140.341089559055844No Hit
GAGATGGAGAAGTTCCATTTGGTCAGTTGATGTGTGTGTGAGTGTGAGTT8050.33731829857488255No Hit
GAGAGAGTGAGCGAGTGATCGAAACAGCAAGTGTGTGAGAGAGAGAGACA7910.3314518933822759No Hit
ATTTGCTGTCAACTTTGTGAATCATCCTCCAACTCAAGCTCCTCATCGTC7510.31469073568911404No Hit
GCCCATGGTTGTGGGTGAGTGAGTGATCCTGTACTGTCGAGAAAAGCTTC7510.31469073568911404No Hit
ATCTGTATAGCAGCCCTTGCGGGAAAGTACGTTTCCGTACCGGTCAACTA7500.314271706746785No Hit
AAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA7420.31091947520815266No Hit
AAAGACTCTTGCGAGAAATACCCTTTCAGATACTGTCCTTTACACTTCGA7350.3079862726118493No Hit
CTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGGTGCACGACAGC7140.2991866648229393No Hit
GATTGAGCACCGCATAGCGCTGCACCAGCTGGCCGCCGCCGGAGTGGCCG7010.2937392885726617No Hit
CAACAGATGATGCATTTTTATCTTTTCACATTTTCGCCACTTCACATTTT6970.2920631728033455No Hit
GGGGTCAGCAGCCAGTGGAGCACGCGCTTGTAGTTGGCGTCGGTGGCCTG6840.2866157965530679No Hit
ATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT6810.2853587097260808No Hit
GGCGGGGCTTGCTGCTGCTGCTGACTCGTCACTGCCAGATTGGAAGAGTT6510.2727878414562094No Hit
GAAGCGCTAGGCCATGTGGTTGGCGGTCAGGATGTTGGTGGCGCCGGCCA6070.25435056799373135No Hit
GGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCAC6060.2539315390514023No Hit
GCGGCCACCAGCGCCCGCGCCAGGGCTTCAAGCCGATCCACATGCCGAAG6020.2522554232820861No Hit
GGAAAAATTTTCATCTCTAAATGAAAAAAAACTTTCGTGTCTTCATTTCG5960.24974124962811184No Hit
CCCAATACTCGAACCACAAACAAGAGACACTTATAGAGAATGCGTTAACG5860.24555096020482137No Hit
CACCGCCTGGGGAGTACGCCCGCAAGGGTGAAACTCAAAAGAATTGACGG5850.24513193126249228No Hit
CCAGGCCAAGCAGCAGGCGGCGCGCTGCATGGACTGCGGCATCCCGTTCT5570.233399120877279No Hit
TACAGAAGGTGGCGGACGAATCAGAGCTGCGGACCCATCAAACCCACCAT5550.2325610629926209No Hit
CAATAAAACATATCCGAAGTTTATTTTGCGTAACATCATATCACAGTAGC5540.23214203405029188No Hit
CAGGTTTAGAGGCCGTCATCGGTGTCGAGCGTGTAGCCCATGCCGCGCAC5420.2271136867423433No Hit
GTCAAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCT5340.22376145520371094No Hit
GCGAGCGACCGAGCGCAGGCAGCACCGATTGCAGCGCGGTGTTGCCCGCA5330.22334242626138187No Hit
AGGGCTGCGGCTGAAGTTGAATCCTCTGCCGTCGGTTATCGAGGGCCAGC5280.22124728154973664No Hit
AATAGATCGAGGACTTAACCCAACATCTCACGACACGAGCTGACGACAAC5160.21621893424178806No Hit
CCAGAAGAAGCTGGCCGACATGCAGACCGAGATCACGCTGGCCCTGCAAG4940.20700029751054908No Hit
GCGTACCGCCTGTGCCGCCGGGATTGGCCGCTTGTTCGCTTTCGCCGGCA4910.2057432106835619No Hit
GTAATGACGAAGATGATGGTTGTTCTGTAGGGAATTAAAATTAAAAAATT4910.2057432106835619No Hit
GTCGCACAGGGCAAGCTCGGCGTCGTCAGTGATGATTTCGGCCTTGCCAC4850.20322903702958764No Hit
GGAAGCGGCTCGGCTCGCGCAGATACTCAACCAGTTCAGCAACTTCTTCT4840.20281000808725858No Hit
CCGATAGCCAGCGTCGTTCATCTTCTCTATGACCTCGTCGGCGATGCGGC4690.19652457395232287No Hit
CATGCAGCACCTGTGTTCTGGCTCTCTTTCGAGCACTCCCAAATCTCTTC4610.1931723424136905No Hit
GTCTCGCCCAGCTCCATGAAGCCGGCCGGCAGCGTCCACAGGCCGTAGCG4580.1919152555867034No Hit
GGTACATGGCTTCTGTGGGCTGCATAGGGCAGACAAAGGGCCGGCTTCAG4520.1894010819327291No Hit
CTTGCTGCTGAAAGACCTGTACGCTCGCCGGACGTATACCGCAATTCTCG4520.1894010819327291No Hit
CGGAAACGTACTTTCCCGCAAGGGCTGCTATACAGATGCTGCATGGCTGT4480.18772496616341292No Hit
GTGCGCAATCCTATTTGTCATAATCAGCACGCTTACCAAAGGGGTAAGTC4360.18269661885546434No Hit
GGAAGATGGTTGAGCAAGATGAAATTTAAAATTATTGATTTTTCCCTTTG4220.1768302136628577No Hit
GTTACCCGGGATCTTCCGCTATATGCCCTAGTGATGCCTCTGGCGGAGGC4220.1768302136628577No Hit
ATTTTCAGCAAGCTGTTTGTGCTGAAGTCAGTCGCGTGATTGGAAGACGA4190.17557312683587054No Hit
CCCGCCATTGTCGCGTTGCTGAGGGCCAAATGGGCGCGCGAAGAGGAGTA3980.16677351904696056No Hit
GGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAG3940.16509740327764438No Hit
CCCGACGGGTCGGGAGAGAGCAGGGTCGTGCTCAAGCTGCATCCGCGCAT3900.1634212875083282No Hit
AGGTATTCTGTGGGGTATTCGGCCTGTTTGTATGGGGATGATGCTTGGAA3880.1625832296236701No Hit
GCGAGATCGTCGTCAAAGCCTTCGATCGAGGCGATTTCGTCGACTTCGAC3710.15545973760407633No Hit
GCTCCAGGTTCTTCTCTTCGAGGGCACGCTCAAAGCCTTTGCGTTGTGCC3700.15504070866174727No Hit
CCGCACGCAATCCGTCCTCACCACCGGCGACGATGCGCTCAAGCTGTGCA3670.15378362183476013No Hit
GAAGACGAAGAGAGCGGACGTTAGCCGGAACCGCCGTAAGCTAGAGTCCG3640.152526535007773No Hit
ATCATGACCGGCGACCCGGTCACACCGTTCATGGTCGACCTGTGGCGCTT3620.1516884771231149No Hit
CGAGGATGGTCAACTGCATGGATTCACTCTGCCACGAACGGCCGCGAAAT3580.1500123613537987No Hit
ATCGTGTCGCCATCGTTCGATGACGGCTTACGGAGGTTGAGCTTGAGCCT3540.14833624558448252No Hit
CTTCGCGCTTCTCTTTCTTCGACTCGCTCCCATTCTTCAGTGAACCCGTG3540.14833624558448252No Hit
ACTGACGACCCACGACGAACTACACCTGCGCGGAAGGCCAGCGCTCACAG3530.14791721664215346No Hit
CTCGCTCAAGAAGCGCAGTACCTCGCGTAGGAGTTCCTGCTTGAGAAGCA3530.14791721664215346No Hit
GGCCAATCCTGCGGCAGCAGAACCACAAGGCCCCCACAACCCCGCACATG3520.14749818769982442No Hit
GCTTCCGTCGCGGCCCCACTCCGAGAGACCAAAGACTCCGGGCGCGGCGC3500.14666012981516635No Hit
GAGGACGGCGAGGACAGCGTGCAGGCCAGCGGCCGACCGGCGCCGGTCAG3430.14372692721886302No Hit
TCCACGGTCACGCTGGACGGGGCGACTCTCTCACGAGCATCCGAGCTCGC3390.14205081144954682No Hit
GGAGGTTCCGAGGTGGGTACGGTAGGAGCCGCCGCTAGCCACAACTCAGC3380.14163178250721778No Hit
CAAGGCGGTCGCCTTCTACGAGGAGCTGGACTGCCGTGTCGAGCGTCGGA3360.14079372462255968No Hit
GCTCTTTCAGCTGGGAAGATAATGACGGTACCAGCAGAAGAAGCCCCGGC3350.14037469568023064No Hit
TCTTGGGAGTGTTTTTGATGCTTCTGGGGTGGGTGAGTGTGTGAACCGAT3330.13953663779557254No Hit
GCGGATTAATTCGATGCAACGCGAAGAACCTTACCAAGGCTTGACATGTT3250.13618440625694017No Hit
GCGAGGCCGCCGGCGTCTACCGCGCGCCCGAGCAGATCGCCATGGTGCAG3240.13576537731461114No Hit
ATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTA3180.13325120366063684No Hit
AATACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACG3090.12947994317967543No Hit
GAGGCGGCCTGGATCTCCTCTCGCACCGGCCTTCCCACCGTGAGCAACCT3080.1290609142373464No Hit
GAGTAGCCGTCGAGGGTGGTGGCGGCGTCCTTCATGTGGACGTGGTAGAT3060.12822285635268826No Hit
CGATGGGCTCGAGCGTCTAGATGGTCGAGGAGGCCGCGCCGGACTTGACG3060.12822285635268826No Hit
CGACGGCGGCGTCGCCGCGATCGAGCTCGGCGGGCTCCGCCTGGGCCTGC3030.12696576952570116No Hit
CGATATTGCCGCGCACGTCCAGAAACCCCAGCAACCCCCCGGTCTGCGGC3020.1265467405833721No Hit
CCAGCTTGCATCAGTGAAGAAATGCGGCCACATGGGTGGCAAGGTACTGG3010.12612771164104306No Hit
CTCCCCGACGGCGACTCGCACAGTGCGCGTTCGTGGCCCGTCGAACTCTG3010.12612771164104306No Hit
GCCACGCCTGAAGCCAGCTGCCTCCTAGGTTCCTCACTTGCATGCACTAC3010.12612771164104306No Hit
GGGACACGGCGACTACGCCACCAACGTCGCCCTGCAGCTCGCGAAGCAGG3000.125708682698714No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTAT2980.12487062481405592TruSeq Adapter, Index 27 (97% over 39bp)
TTTTATTCCCTTCGGTGGAAAGGGTTTCGTTGAGCAGCTCCAGCTTTTCC2970.12445159587172686No Hit
GGCCCTGCCGCTGGTGGCCATCCTGGCCAAATCGGTCACCGACGCCAATG2940.1231945090447397No Hit
GCGACTACCTGTTCGCCCGCAGCCGTGGCCGCAGCGCGCCGGTGAAGACC2930.12277548010241068No Hit
GAGCCTGACAAGAACACCTCCGCTGGGGACACAGGCCTAGCTGAGTGGTG2920.12235645116008163No Hit
TGGCTGAGCTGACCGGTCGCGCTGCAGGCATCAGCCGCGGCAAGGGCGGG2920.12235645116008163No Hit
GTCTCCACTGAGTCCCCACCATAACGTGCTGGCAACAGTGAACAAGGGTT2890.12109936433309448No Hit
CGCCCAGCGAGTGACCTGGAAAAACCTGATTGTGTGCCCGGGCCTGCGCC2860.11984227750610735No Hit
CCCCGCATAGAGGGCGAGCTTGCATTCCGCCTCGGCGAAAGCCTCTCCGG2830.11858519067912021No Hit
AAAAAAACAGTTTAAGACGAAAAGTGCAAAGTAACATCTTCAGTAATTGT2810.11774713279446211No Hit
AGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGAGTTAACCTCG2770.11607101702514594No Hit
GGCCGGTGTATTTTTTTGCCAGTTTCAGTGCGGCTTCTACCGCATCTGCG2720.1139758723135007No Hit
GGGCTACGCCATCGGCAACGGGGCGGCCTGCTCTTCTTCGGAGCACAAGG2710.11355684337117165No Hit
CGTGTCTTCACTTTTAAGGACATCCGATGCGAAAATGTTTCCCACAACAG2680.1122997565441845No Hit
AGTTCCTCGGCCTTGTTCAGCGCGGCGCGAGTGGCATTGGCGTCCGACTC2650.11104266971719737No Hit
GGTCGGGCTTGGCGAGCGGGGCGCGCTCCGACAGTATCCAGCGGCTGATC2650.11104266971719737No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT2640.11062364077486832TruSeq Adapter, Index 27 (97% over 39bp)
CAATATATAAAATAGGTACTTTATTTTTCAATTCAATTCGCAAGGGTGTC2630.11020461183253927No Hit
GTGCTTACAAGCCGTCGGAGCCTAAGGCCGCACTTTGTGTGGTTGGTGAC2610.10936655394788118No Hit
CTTCGGGGGCAGGGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTC2600.10894752500555213No Hit
GTTTTAATTGATTTTAAATTCTAAATTTCTCCTTGCAGTGAAATCTGCCT2580.10810946712089403No Hit
GCGAGCGATCGCCTGGTCGAGACCGGCGCGCACTTTATAGAAGCCTTCCT2560.10727140923623595No Hit
GAACACCGGTGGCGAAGGCGGCTTTCTAGCATATGCCTGACGCTGAGGCG2530.1060143224092488No Hit
GGCCGCCCTCGACCCCGAAGAGAACGCCGAGGACGACTGGCAGGGCGGTG2480.10391917769760356No Hit
GTCCAATCTTTACATTCGCCGGTCAGCTGCGGATGGACACGCCACTAACA2470.10350014875527454No Hit
AAGGGCCATGATGACCTGACGTCATCCCCACCTTCCTCCGGTTTGTCACC2420.10140500404362929No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT2390.10014791721664215TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT9900.0145.027475
ATTCAGC306.475602E-10145.027476
CTAATGT151.2279139E-4145.027476
ATTAGCC202.1252436E-6145.027456
GGGCTAC253.698551E-8145.027451
CGACCCA253.698551E-8145.027456
GTTAGGA100.007083492145.027451
TCCTAAC100.007083492145.027452
ATCGTGT351.0913936E-11145.027451
GGCCAAG351.0913936E-11145.027454
TCGTGTA100.007083492145.027452
CCACTGA253.698551E-8145.027455
AGGCACT100.007083492145.027452
GGCTGAG400.0145.027452
CCACATA1800.0145.027455
ACAGTCT1750.0144.997077
TAGGTAT100.0070879324144.997077
CCTAATA151.2289398E-4144.997077
GTAGCGT151.2289398E-4144.99707145
ACGGAGC151.2289398E-4144.997077
Produced by FastQC (version 0.11.5)