FastQCFastQC Report
Wed 2 Jan 2019
Edm-A_S31_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEdm-A_S31_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625031
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81734322.54720028601135No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3319099.15603204496734No Hit
GCATCCTGGCAGCAAAATGATGTCTGGATGGAAGGCTTCGTTTGTATATA1428893.941731808638326No Hit
GTTTGCTCAAAAAGTGCGGCTCGATAGGACGCGCGGCGAGACTATGCCCG937892.5872606330814825No Hit
CTGGGCTCCATGGTTGCACTAAACGCCTTCCTGAGTACACAGCCCGCCAA397391.0962389011293971No Hit
GGCGGATGAGCTGGGAGTAGACGGCCTGGTCGTTGTAGAAGCTCTTGGAG387811.0698115409219948No Hit
CGCGGATGCCCAACAAGTTGCTCAGGCGGCTGTGGGTGAAGTCCACCCCG324820.896047509662676No Hit
CGTACTTCCTGGAACGGCGCGGGCGCCGGGGGGCTGAAACGCGAAACATC283050.780820908841883No Hit
GCGGCCGCTGCGGCCGCGTCCAAAACCTCTACGGAGGTGCAAACGGACCC251880.694835437269364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241280.6655943080210901No Hit
GTGGTGGTTCGGCGTCGGGTAGTCGCTCGTCTTCTGGGGCGGTAGGTGTA235460.6495392729055283No Hit
GTGGAGCACTACTTGAAAGTGGGCAGGCAGAACGGGGTTCTGGAAAGCGA231330.638146266887097No Hit
AGAGCTTCTACAACGACCAGGCCGTCTACTCCCAGCTCATCCGCCAGTTT228430.6301463353003051No Hit
TCCATCGCGCCTACCCCCGGCTATCGTGGCTACACCTACCGCCCCAGAAG225500.6220636458005463No Hit
GCCGTTCCAGGAAGTACGGCACCGCCAGCGCACTACTGCCCGAATATGCC213140.5879673856582192No Hit
GCGGGTTCCAGTGGTGGTTCGGCGTCGGGTAGTCGCTCGTCTTCTGGGGC197600.54509878674141No Hit
CAAACGTCCCCGGTTGCCTCGGCGGTGGCAGATGCCGCGGTGCAGGCGGC175840.48507171386948134No Hit
GCCGCCTGCACCGCGGCATCTGCCACCGCCGAGGCAACCGGGGACGTTTG173940.4798303793815832No Hit
GATGGTTTTCAGGTCGCCGCCCCAGTGCGAACGGTCGTCAAGATTGATGG155210.42816185571930276No Hit
CGAACGGTCGTCAAGATTGATGGTCTGTGTGCTTGCCTCCCCCGGGCTGT152690.4212101910300905No Hit
GCATATTCGGGCAGAAGTGCGCTGGCGGTGCCGTACTTCCTGGAACGGCG151110.4168516076138383No Hit
GCTGATCTGGCTCGTGGAGCGGAAGGTCACGGGGTCTTGCATCATGTCTG117810.3249903242206756No Hit
GCGGGGGTAAGCGCGATGGAAGGATGTAGGGCATATTCGGGCAGTAGTGC117030.3228386184835385No Hit
GCGGCATCTGCCACCGCCGAGGCAACCGGGGACGTTTGTGTCTCCATGCC101960.2812665602032093No Hit
CGGCAATGCAGCCGGTGGAGGACATGAACGATCATGCCATTCGCGGCGAC88880.24518411014967872No Hit
GCGTCTGGCGTCAGTAATGGTCACTCGCTGGACTCCTCCGATGCTGTTGC84880.23414972175410362No Hit
GCGTTTCTTGCTGTCCTCTGTCAGGGGTTTGATCACCGGTTTCTTCTGAG83560.23050837358356385No Hit
GCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77680.21428782264206842No Hit
GCCACAGAGGGCATGGAGACACAAACGTCCCCGGTTGCCTCGGCGGTGGC70070.1932948987194868No Hit
ATGGACCCTCCTTTGCACTCCTGACGTAACCTGCGGCTCGGAGCAGGTCT67920.1873639149568652No Hit
GAGGAAGAATGCACCGTAGGAGGGGCATGGCCGGCCACGGCCTGACGGGC65740.18135017328127676No Hit
CGGGATTCCGAGGAAGAATGCACCGTAGGAGGGGCATGGCCGGCCACGGC64960.17919846754413962No Hit
TTTTGAGCAAACATGTCCATCCTTATATCGCCCAGCAATAACACAGGCTG62870.17343299960745162No Hit
GTGGGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61330.16918476007515523No Hit
GGTACCCAAGCGCCAGCGACTGGAAGATGTCTTGGAAAAAAATGACCGTG55640.15348834258244964No Hit
GGCAAGCACACAGACCATCAATCTTGACGACCGTTCGCACTGGGGCGGCG50080.13815054271260024No Hit
GCCAAGTCACCAGACTCGCGCTTTAGGCCGCGCTTTTCTTCGGACGGTGC41950.11572314829859387No Hit
GGGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41270.1138473022713461No Hit
CTGGGTGAAGGTGCTGTCATTGCTTATTAGGTTGTAACTGCGCAACCCGA40620.11205421415706515No Hit
GCAGCAGGTGCTGCCCACGCTTGCACCGTCCGAAGAAAAGCGCGGCCTAA37780.10421979839620682No Hit
CGGGAAGTCAGCGTGCGGCACCGGGTTCGGGTACACACCCGCGGCGATCA37490.10341980523752763No Hit
ATGGTACCCAAGCGCCAGCGACTGGAAGATGTCTTGGAAAAAAATGACCG37410.10319911746961613No Hit
GGCGACGGCGACGGCGGCGGCGGGTTCCAGTGGTGGTTCGGCGTCGGGTA36460.10057845022566703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAA101600.0140.685076
TGCTCAA101550.0140.613544
GCTCAAA102000.0139.991265
CTGCCTG155000.0139.76472145
TGGGCTC48650.0134.122712
GCGGATG81050.0132.925552
GTTTGCT107400.0132.741471
GCTCCAT49700.0132.131595
TTTGCTC107900.0131.899022
TCAAAAA108950.0131.792927
ATGCCCA35200.0131.580966
CGGATGC35200.0131.376853
GGGCTCC50050.0130.630113
CAAAAAG110050.0129.621158
TTCTACA28050.0128.944416
TCCATGG51400.0128.466557
GGCTCCA51400.0127.904254
CTTCTAC28400.0127.100085
TCAGGTC19000.0126.286579
CGCGGAT37150.0125.574221
Produced by FastQC (version 0.11.5)