FastQCFastQC Report
Wed 2 Jan 2019
Edm-A_S31_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEdm-A_S31_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3625031
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89611424.72017480678096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48850213.475802000038067No Hit
GCTGCCCGAGGGCAACTACTCCGAGACCATGACCATAGACCTTATGAACA1450244.000627856699708No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT1356483.741981792707428TruSeq Adapter, Index 6 (97% over 36bp)
CAGATTTTGGCGCGCCCGCCAGCCCCCACCATCACCACCGTCAGTGAAAA960142.648639418531869No Hit
GTTTGCTCAAAAAGTGCGGCTCGATAGGACGCGCGGCGAGACTATGCCCG838852.314049176407043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT611721.6874890173352999TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG459861.2685684618972914TruSeq Adapter, Index 6 (97% over 36bp)
TGAAAGCCTGGCTCAGGTTTACGGTCTGCAGGCCTTGTCTACTGGTCTGG413281.1400730090308193No Hit
GCCAGATCAGCAACTTTCCGGTGGTGGGCGCCGAGCTGTTGCCCGTGCAC365021.0069431130382058No Hit
CGGGGTTCTGGAAAGCGACATCGGGGTAAAGTTTGACACCCGCAACTTCA345320.9525987501899983No Hit
GCAGACCGTGGACGTTCAGATACCCACCACCAGTAGCACTAGTATTGCCA287980.7944207925394292No Hit
GCGGGTTCCAGTGGTGGTTCGGCGCCGGGTAGTCGCTCGTCTTCTGGGGC261950.7226145100552244No Hit
GGGCGCGCCAAAATCTGGTTCTCGGGAAAGCGATTGAAACGTGGGTCAGA237310.6546426775384817No Hit
CGCGTTTCAGCCCCCCGGCGCCCGCGCCGTTCCAGGAAGTACGGCACCGC236010.6510565013099199No Hit
GCGACGGCGACGGCGGCGGCGGGTTCCAGTGGTGGTTCGGCGTCGGGTAG235480.6495944448475061No Hit
GGCATGACAAGACCAGTGACTGGGTCAAACCCCAGTCTGAAGTTGCGGGT235220.6488772096017937No Hit
CCGAATATGCCCTACATCCTTCCATCGCGCCTACCCCCGGCTATCGTGGC209850.5788916012028588No Hit
GCCACCCTGCGCACGGAAATCGGGGCCAGCACGGGCTGGCGACTGCGACG205890.5679675566912393No Hit
GGCATATTCGGGCAGTAGTGCGCTGGCGGTGCCGTACTTCCTGGAACGGC179260.4945061159476981No Hit
GCGACTGGAAGATGTCTTGGAAAAAAATGACCGTGGAGCCTGGGCTGGAG176740.4875544512584858No Hit
ATCGCGCCTACCCCCGGCTATCGTGGCTACACCTACCGCCCCAGAAGACG166090.4581753921552671No Hit
CTTGACCACAGCAACTTTCTAACCACGGTCATTCAAAACAATGACTACAG159080.43883762649202174No Hit
GGCAAGCACACAGACCATCAATCTTGACGACCGTTCGCACTGGGGCGGCG157450.43434111322082486No Hit
GGGCACTACCGCGCGCCCTGGGGCGCGCACAAACGCGGCCGCACTGGGCG151720.41853435184416354No Hit
CAAAACCTCTACGGAGGTGCAAACGGACCCGTGGATGTTTCGCGTTTCAG122320.3374315971366866No Hit
GTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGAGCC119810.33050751841846315No Hit
AGCAGGTCTACTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTC113540.31321111460839923No Hit
GTCCCCGGTTGCCTCGGCGGTGGCAGATGCCGCCGCCGTGCAGACCGTGG105550.2911699237882379No Hit
GCGGTACTGGGTGAAGGTGCTGTCATTGCTTATTAGGTTGTAACTGCGTT91990.25376334712723836No Hit
CCTGCTCTCACAGATCACGGGACGCTACCGCTGCGCAACAGCATCGGAGG87350.24096345658837126No Hit
GCAACCCGAGGTCGAGAAGCCTCAGAAGAAACCGGTGATCAAACCCCTGA86780.2393910562420018No Hit
GAAACATCCACGGGTCCATTTGCACCTCCGTAGAGGTTTTGGACGCGGCC72370.19963967204694252No Hit
GTCACGGGGTCTTGCATCATGTCTGGCAAACCAGTAGACCTGCTCCGAGC70030.19318455483553104No Hit
CTTGCCTCCCCCGGGCTGTAGTCATTGTTTTGAATGACCGTGGTTAGAAA67830.18711564121796476No Hit
GATTAATGGTCTGTGTGCTTGCCTCCCCCGGGCTGTAGTCATTGCTTTGA66090.1823156822658896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTG62720.17301921004261755TruSeq Adapter, Index 6 (97% over 36bp)
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.16226068135693184No Hit
GCGCCCAGTGCGGCCGCGTTTGTGCGCGCCCCAGGGCGCGCGGTAGTGCC58210.16057793712660665No Hit
GCTTGATTGGCCGCACGCGGACCTCGGGCTCCAGCCCAGGCTCCACGGTC57250.15792968391166862No Hit
GATGGTTTTCAGGTCGCCGCCCCAGTGCGAACGGTCGTCAAGATTGATGG52040.14355739302643206No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.11793002597770888No Hit
GTTTGCCTACGGAAAGCGGCATAAGGACATGTTGGCGTTGCTGCTGGACG42670.11770933820979738No Hit
ATTAGGTTGTAACTGCGTTTCTTGCTGTCCTCTGTCAGGGGTTTGATCAC41900.11558521844364916No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT39470.1088818274933373TruSeq Adapter, Index 6 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38920.10736459908894573No Hit
AGCTGATCATCGCTGGCGCATCGGCATTCGCGCTGCGCATCGATTTCGAA38170.1052956512647754No Hit
CGCACGCGGACCTCGGGCTCCAGCCCAGGCTCCACGGTCATTTTTTTCCA37960.1047163458740077No Hit
GTGGCTACACCTACCGCCCCAGAAGACGAGCGACTACCCGACGCCGAACC37790.10424738436719576No Hit
GCGCACTACTGCCCGAATATGCCCTACATCCTTCCATCGCGCCCCCCCGG37700.10399911062829532No Hit
AGGCTCCACGGTCATTTTTTTCCAAGACATCTTCCAGTCGCTGGCGCTTG37600.10372325091840594No Hit
TGGCACCCACCGTGCAGCTGATGGTACCCAAGCGCCAGCGACTGGAAGAT36750.10137844338434622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTTT101600.0142.661682
CAGATTT102150.0142.108461
CTCAAAA91150.0141.35956
TGCTCAA91400.0140.893514
GATTTTG102900.0140.86133
GCTCAAA91550.0140.662675
ATTTTGG103150.0140.519884
TTGCTCA92450.0139.450183
CAAAATC26000.0138.864219
CATGACA26450.0137.342933
AGGGCAA162100.0136.679929
CGAGGGC162700.0136.175867
ATGACAA26750.0136.073684
AGGTCTA12000.0135.955114
TTGGCGC106350.0135.795757
CCAAAAT26600.0135.731938
GACAAGA27000.0134.813756
TCAAAAA95350.0134.810077
GTGATCT94650.0134.277145
CAAAAAG96100.0133.98438
Produced by FastQC (version 0.11.5)