FastQCFastQC Report
Wed 2 Jan 2019
Ebiom-R_S32_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEbiom-R_S32_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17744381
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10289385.798669449218882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9647285.436808418394532No Hit
GGTCTATGGTCATGGTCTCGGAGTAGTTGCCCTCGGGCAGCGTGAACTCC2054251.1576904260565641No Hit
GCGGCGTCTGGCGTCAGTAATGGTCACTCGCTGGACTCCTCCGATGCTGT1278110.7202899892647706No Hit
GTGAACTCCACCCACTCATATTTCAGCTCCACCTGTTTGTCCTTAGTAAG1204630.6788796971841397No Hit
AGTCTTTCTACCCTTGGGAATGGGGAGAAAAAGGAATCTCTACCCCAAGA944390.5322191853297109No Hit
AGATTTTGGCGCGCCCGCCAGCCCCCACCATCACCACCGTCAGTGAAAAC925210.5214101297757301No Hit
GTTCAATCGCTTTCCCGAGAACCAGATTTTGGCGCGCCCGCCAGCCCCCA914370.5153011536440747No Hit
GGTCACTCGCTGGACTCCTCCGATGCTGTTGCGCAGCGGTAGCGTCCCGT910590.5131709018195676No Hit
GTCTGGCGTCAGTAATGGTCACTCGCTGGACTCCTCCGATGCTGTTGCGC822930.463769347603616No Hit
CTCTGACCCACGTGTCCAATCGCTTTCCCGAGAACCAGATTTTGGCGCGC751640.42359324904035817No Hit
TCACCCAGTACCGCAGCTGGTACCTTGCATACAACTACGGCGACCCTCAG746390.4206345659507649No Hit
CTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGA745360.4200541005065209No Hit
GCAAGGAGAGACAAGAGGGTCATTGAAGTCGGAGTCAGCGTGGATCGGCC730290.41156127114267893No Hit
TGAACACGTGGGTCAGAGAGGTAAACTGGCGGATGAGCTGGGAGTAGACG700900.394998281427794No Hit
CAAAATATGGTTCTCGGGAAAGCGATTGAACACGTGGACCAGGCCGTCTA688820.3881904925283108No Hit
GTGTTGCCAATTAAAAGTTGAATCAATGCAAGTAAACAATCTGCAGTTTT654320.3687477179395551No Hit
CTGCATACCAACATGCCAAATGTGAACGAGTTCATGTTTACCAATAAGTT624890.35216218587732084No Hit
CGGGACGCTACCGCTGCGCAACAGCATCGGAGGAGTCCAGCGAGTGACCA604530.3406881310765363No Hit
GGTTTACCTCTCTGACCCACGTGTTCAATCGCTTTCCCGAGAACCAGATT593900.3346975022684646No Hit
GCACGCGCACTGGGTGTTGGTCGGAGCGCTTCTTTGCCCCGCCAAACATC547380.3084807523012496No Hit
GGGTAGTCGCTCGTCTTCTGGGGCGGTAGGTGTAGCCACGATAGCCGGGG486450.2741431217014558No Hit
CGGGTAGTCGCTCGTCTTCTGGGGCGGTAGGTGTAACCACGATAGCCGGG481590.27140422649851803No Hit
GAAACATTAAAATTAGAAGGAAAAGGTCCAGAATTAGTGGGGCCATCAAA474680.26751003599392953No Hit
GCAGGTGGCACCGGGACTGGGCGTGCAGACCGTGGACGTTCAGATACCCA450370.25380992439240346No Hit
GCTTATAAACCCTCTGCCTTGAAGGAATACGCATTCCTGCTACCCCTATT447620.2522601380121403No Hit
CTCCAGTAAATAATTGATGAGTTCTCCTCAAAATAACTCAAATGGCTCAA437140.2463540430066284No Hit
GTGCAAAGGAGGGTCCATGAGCGGATCCCGGTCTGAGGGTCGCCGTAGTT397660.2241047461728871No Hit
GAACGGCGCGGGCGCCGGGGGGCTGAAACGCGAAACATCCACGGGTCCGT396470.22343411133924596No Hit
CGGAAAGTTGCTGATCTGGCTCGTGGAGCGGAAGGTCACGGGGTCTTGCA392270.2210671648675713No Hit
GCGGCTCGGAGCAGGTCTACTGGTCGTTGCCAGACATGATGCAAGACCCC391010.22035708092606895No Hit
CGCGGTGCAGGCGGCCGCTGCGGCCGCGTCCAAAACCTCTACGGAGGTGC384980.21695882206316466No Hit
GATAGCCGGGGGTAGGCGCGATGGAAGGATGTAGGGCATATTCGGGCAGT339350.19124363932447122No Hit
GAATGCGGCACACACCACCCAAATGACAGGAAGGCAATGCGTTGCGGTGG339050.1910745717193516No Hit
AGCTCTGCGGGAACGATGAGGTCTGTGGGTTTTGCTGCTGCTGTTGCTTT320190.18044585494416512No Hit
GCGGCATCTGCCACCGCCGAGGCAACCGGGGACGTTTGTGTCTCCATGCC310970.17524984388015563No Hit
GGGGTAGGCGCGATGGAAGGATGTAGGGCATATTCGGGCAGAAGTGCGCT307070.17305196501360065No Hit
GCGGCGGGTTCCAGTGGTGGTTCGGCGTCGGGTAGTCGCTCGTCTTCTGG303650.17112459431523702No Hit
CGATGCTGGGGTGGTAGCGCGCTGTTGGCAGCACCAGGGTCCTGCCTCCT301350.1698284093426533No Hit
AAAAAAATGACCGTGGAGCCTGGGCTGGAGCCCGAGGTCCGCGTGCGGCC295590.16658231132435672No Hit
CCGGGACTGGGCGTGCAGACCGTGGACCTTCAGATACCCACCACCAGTAG289500.16315023894042852No Hit
CGGATGAGCTGGGAGTAGACGGCCTGGTCGTTGTAGAAGCTCTTGGAGTG289420.16310515424572997No Hit
GTTCTCGGGAAAGCGATTGAACACGTGGGTCAGAGAGGTAAACTGGCGGA277890.15660732262229943No Hit
CTGGCGACGGCGACGGCGGCGGCGGGTTCCAGTGGTGGTTCGGCGTCGGG277080.15615084008847646No Hit
AGTAGACAAGGCCTGCAGACCGTAAACCTGAGCCAGGCTTTCAAGAACTT273890.15435308788737123No Hit
GCCACTGCCACAGAGGGCATGGAGACACAAACGTCCCCGGTTGCCTCGGC273310.15402622385080664No Hit
CTGGGAGGTCAGAGGGACCTCAAGATGCAGAGCTGAGCCTGTCAGGACAG266900.15041381268808418No Hit
GTTGTATGCAAGGTACCAGCTGCGGTACTGGGTGAAGGTGCTGTCATTGC252030.14203369506098862No Hit
GCGGCCGCTGCGGCCGCGTCCAAAACCTCTACGGAGGTGCAAACGGACCC242320.13656154024195039No Hit
GTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGAGCC237760.13399171264413223No Hit
GCCGCCTGCACCGCGGCATCTGCCACCGCCGAGGCAACCGGGGACGTTTG235440.13268425649787388No Hit
GGCTTTTAAACGATGCTGGGGTGGTAGCGCGCTGTTGGCAGCACCAGGGT234180.1319741725563715No Hit
GCACGGAAATCGGGGCCAGCACGGGCTGGCGACGGCGACGGCGGCGGCGG234080.1319178166879983No Hit
ATAGCCGACTGGAAACCACTGACTGAAAACCACAGAGTCAGCTCACGGAA233070.13134862241742895No Hit
GCGGAAGGTCACGGGGTCTTGCATCATGTCTGGCAACGACCAGTAGACCT231060.13021586946312752No Hit
GGCCCGCCGCATCCCCGGCCTCAGCCGGTTCATTGCCGAACCAGAACGCA228610.12883515068798398No Hit
CGCTGCCTTTGAGCTCTTATAAGATTTCGCGCTACATATAATTAGCTATT222660.1254819765197783No Hit
GCGAAGATGTCTTGGAAAAAAATGACCGTGGAGCCTGGGCTGGAGCCCGA216410.12195973474645298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209450.1180373663076779No Hit
CGGACACCATCACCCGCGCTTAAACTTATTGGTAAACATGAACTCGTTCA208370.1174287229292473No Hit
ATTGCTCATGGACAGAGACTCATTCCTTATGATTGGGAGATTTTGGCCAA197830.11148881440271148No Hit
GCGGCCGCAGCGGCCGCCTGCACCGCGGCATCTGCCACCGCCGAGGCAAC194410.10956144370434787No Hit
GGCGACCCTCAGACCGGGATCCGCTCATGGACCCTCCTTTGCACTCCTGA191170.10773551356905603No Hit
ATTCTAGAGAAAGATAATAGAATGGGCAATGATATAAAGACACAACAACT187500.1056672531997594No Hit
ATATAGATACAGATACAGATACAGATACAGATACAGATACAGATACAGAT187020.10539674503156803No Hit
GTAAAGCCCCCTTATATGCTAGTTGTAGGAAATATAGTTATTAAACCAGA185710.10465848315587903No Hit
AAATACCAAGGCTTGAATGGTACCGAAATTGCCATCTCTGAATCTCAAGA179890.10137857161655851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAT259000.0117.49351
TATGGTC275700.0110.1875845
CTATGGT280500.0108.3026354
GTCTATG291700.0104.0993652
CGTCTGG191200.0102.207735
ACGCGCA79700.0101.135383
TCTATGG306700.099.075023
GCGTCTG199700.097.857934
GGCGTCT200600.097.527843
TGGTCAT315550.096.480657
TACCCTT160600.091.362029
GGTCATG338550.089.799128
GCGGCGT219250.089.187581
TAGTTCG28750.087.75864145
GCATACC105350.087.3827743
GTCATGG348150.087.3506859
TACCAAC106850.086.155346
AGTACCG136600.085.434697
ATGGTTC158350.084.960887
GGTTTAC108800.083.495031
Produced by FastQC (version 0.11.5)