FastQCFastQC Report
Wed 2 Jan 2019
Ebiom-R_S32_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEbiom-R_S32_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17744381
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14880038.385770120693419No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10805426.08948827237197No Hit
CTGCATACCAACATGCCAAATGTGAACGAGTTCATGTTTACCAATAAGTT2106461.1871138249342144No Hit
CTTGACGACCGTTCGCACTGGGGCGGCGACCTGAAAACCATCCTGCATAC1396990.7872858455868368No Hit
CGAGCTGTTGCCCGTGCACTCCAAGAGCTTCTACAACGACCAGGCCGTCT1307540.7368755213270048No Hit
ATATTTTCTACAACTAGCATATAAGGGGGCTTTACGCAACTTTGTAAAGG975590.5498022162621509No Hit
GCGGCGTCTGGCGTCAGTAATGGTCACTCGCTGGACTCCTACGATGCTGT936720.527896690225486No Hit
GTCACTCGCTGGACTCCTCCGATGCTGTTGCGCAGCGGTAGCGTCCCGTG933100.525856607790376No Hit
CTCTGACCCACGTGTTCAATCGCTTTCCCGAGAACCAGATTTTGGCGCGC924430.5209705540024191No Hit
CTGGACTCCTCCGATGCTGTTGCGCAGCGGTAGCGTCCCGTGATCTGTGA874180.4926517301448836No Hit
CTCTGACCCACGTGTCCAATCGCTTTCCCGAGAACCAGATTTTGGCGCGC831640.46867794373892224No Hit
GTCTGGCGTCAGTAATGGTCACTCGCTGGACTCCTCCGATGCTGTTGCGC783430.4415087795962001No Hit
ATTGAACACGTGGGTCAGAGAGGTAACTGGCGGATGAGCTGGGAGTAGAC782710.441103017343913No Hit
TGAACACGTGGGTCAGAGAGGTAAACTGGCGGATGAGCTGGGAGTAGACG769840.4338500170842815No Hit
GGGAGGGTGGGGGGTGCCGGAAACGCTCAGGAGGGGAATGCTTGGGTTGG736100.41483554709516207No Hit
CTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGA716370.4037165342651288No Hit
GTGTAGCCTCATACTACATTAGAATTTGGTCTGGAAATCAAACTTTAGAA665380.3749806769816315No Hit
GCACGCGCACTGGGTGTTGGTCGGAGCGCTTCTTTGCCCCGCCAAACATC621270.3501221034422108No Hit
GGTCTATGGTCATGGTCTCGGAGTAGTTGCCCTCGGGCAGCGTGAACTCC616330.3473381235445745No Hit
GTGATCTGTGAGAGCAGGAACGTTTTCACTAACGGTGGTGATGGTGGGGG612040.34492045679136396No Hit
CGGGACGCTACCGCTGCGCAACAGCATCGGAGGAGTCCAGCGAGTGACCA555600.3131132046815271No Hit
CAAACGTCCCCGGTTGCCTCGGCGGTGGCAGATGCCGCGGTGCAGGCGGC492390.2774906602828242No Hit
CGGAGGTGCAAACGGACCCGTGGATGTTTCGCGTTTCAGCCCCCCGGCGC490300.27631282263382423No Hit
GTTGGGTCTTATTTTCTCTAACCTGCGTTTGAGGTTGTAATGTTACGGGC481820.27153384499577643No Hit
GCAGTAGTGCGCCGGCGGTGCCGTACTTCCTGGAACGGCGCGGGCGCCGG461930.2603246627763459No Hit
CTCTGGATGGTGGCGACTCTGGTGGTGGTTAGAGTTGTCTGTCAAGCGTG460780.2596765702900541No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT443750.25007916590609724TruSeq Adapter, Index 7 (97% over 38bp)
ATTTTATCACTTTGGAACTTTTTATCTGGGTGGTCAAATCCCATTCAACA441650.24889569267025996No Hit
GAGGTCGAGAAGCCTCAGAAGAAACCGGTGATCAAACCCCTGACAGAGGA408920.23045041695170992No Hit
ACCCACCACCAGTAGCACTAGTATTGCCACTGCCACAGAGGGCATGGAGA408790.2303771543228248No Hit
CCAGTACCGCAGCTGGTACCTTGCATACAACTACGGCGACCCTCAGACCG399020.22487118598276268No Hit
GATAGCCGGGGGTAGGCGCGATGGAAGGATGTAGGGCATATTCGGGCAGT396750.22359190777069088No Hit
GGGCTGGCGGGCGCGCCAAAATCTGGTTCTCGGGAAAGCGATTGAACACG396430.22341156899189665No Hit
CGCGGTGCAGGCGGCCGCTGCGGCCGCGTCCAAAACCTCTACGGAGGTGC337590.19025177604110283No Hit
CTGGGAGGTCAGAGGGACCTCAAGATGCAGAGCTGAGCCTGTCAGGACAG327140.1843625877961029No Hit
CCGAGGTCCGCGTGCGGCCAATCAAGCAGGTGGCACCGGGACTGGGCGTG313110.17645585946334222No Hit
CGCACTACTGCCCGAATATGCCCTACATCCTTCCATCGCGCCTACCCCCG312570.17615153777412693No Hit
GCGCACTACTGCCCGAATATGCCCTACATCCTTCCATCGCGCCTACCCCC305500.17216717788014133No Hit
GCGGCCGCTGCGGCCGCGTCCAAAACCTCTACGGAGGTGCAAACGGACCC299570.16882527488561028No Hit
GTTTGCACCTCCGTAGAGGTTTTGGACGCGGCCGCAGCGGCCGCCTGCAC299500.16878582577774903No Hit
CGGGAAAGCGATTGAACACGTGAGCCAGATCAGCAACTTTCCGGTGGTGG299340.1686956563883519No Hit
AGCAGGTCTACTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTC298480.16821099592034233No Hit
GCAGCGGCCGCCTGCACCGCGGCATCTGCCACCGCCGAGGCAACCGGGGA290990.16398994137918926No Hit
CGAATATGCCCTACATCCTTCCATCGCGCCTACCCCCGGCTATCGTGGCT287610.16208511302817497No Hit
GGACACGCTGCCACTGTCCGTGAAGGGCGCTATTAGCAGCAGCAACAGGC277380.15631990769359608No Hit
GCATATTCGGGCAGTGGTGCGCTGGCGGTGCCGTACTTCCTGGAACGGCG276190.15564927285995495No Hit
AGCTCTGCGGGAACGATGAGGTCTGTGGGTTTTGCTGCTGCTGTTGCTTT270790.15260605596780186No Hit
CGCAACCCGAGGTCGAGAAGCCTCAGAAGAAACCGGTGATCAAACCCCTG259690.1463505545783761No Hit
CTACTCCCTCAGGGAGCGGAAGGTCACGGGGTCTTGCATCATGTCTGGCA252610.14236055909755319No Hit
GGGGTAGGCGCGATGGAAGGATGTAGGGCATATTCGGGCAGAAGTGCGCT249650.14069242539370633No Hit
GCCAGCGCACTACTGCCCGAATATGCCCTACATCCTTCCATCGCGCCTAC243700.1373392512255006No Hit
CGGGACTGGGCGTGCAGACCGTGGACGTTCAGATACCCACCACCAGTAGC240090.13530480437722792No Hit
CACCGGGGTGGCGTTGAAGGTGTCGTCCACATAGGTGGCGGCTGCCCGGA235430.13267862091103658No Hit
ATCCTTCCATCGCGCCTACCCCCGGCTATCGTGGCTACACCTACCGCCCC234650.13223904513772558No Hit
AAGTGGCAGTCAGGAGGCAGCCCAGGCCCTCCCATCCGACTTCAGAGGCC234530.13217141809567773No Hit
GGCGACCCTCAGACCGGGATCCGCTCATGGACCCTCCTTTGCACTCCTGA231730.130593453781228No Hit
GCAACAGCAACAGCAACAACAGCAGCAACAACAACAAAAGCATCAACAGC229390.129274726461295No Hit
GGCAATACTAGTGCTACTGGTGGTGGGTATCTGAACGTCCACGGTCTGCA224340.12642875510844814No Hit
AGGGAGCCCGGCCAGTCGTGTTTTTCTCAAAGCCCACCAACAGCAAAAGC208740.11763723964222815No Hit
TCTGCATCTATGTTAACTGGAGGATTGGCAGCCCTATTTTTTTGGATCTG200350.11290898228571625No Hit
GGAAGATGTCTTGGAAAAAAATGACCGTGGAGCCTGGGCTGGAGCCCGAG199450.11240177947035741No Hit
GCGGAAGGTCACGGGGTCTTGCATCATGTCTGGCAACGACCAGTAGACCT196180.11055894257455359No Hit
CAAGTAAACAGTCTGCAATTTTCACAGGTTATAGTTTGGGAGTCTGGTTT191200.10775242032956799No Hit
CCTTGGTCCAAAGGAAAATATATGAAAATACAAAGAGATCCCCCAGTGAA190940.10760589507179766No Hit
CTTATATCCTCAGATCTGTCCTGTACTCTGCTGTTAGTAAGGCTTCTAAA190210.10719449723267326No Hit
ATTGGTGTCAAGGAAACGACGTTCCAAGTCGACAATGATTTCACCATTCC182940.10309742560194125No Hit
AACTAAAGTGCCACTCCTATTTTCAAAACTTTTTATCTTTTTCTTTACTT177670.10012747133867335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG225750.0130.22893145
ACCGTTC170550.0129.439048
GACCGTT175750.0125.650517
TTGACGA180200.0122.5270542
CGACCGT181200.0122.451116
ACGACCG187750.0117.754355
GACGACC189500.0116.4755864
TGACGAC194550.0113.6385353
CCGTTCG195650.0112.833269
CTTGACG198900.0111.483861
ATAGCCG58350.0104.757752
CGATCTA20400.093.82491145
GGCGTCT152200.093.329573
CGTCTGG158550.090.094745
GCCGATC113550.089.07145
TATGGTC104150.088.9060065
GGTCTAT103550.088.7229161
ACGCGCA106700.087.6646963
GTCTATG107300.085.3499152
GCGTCTG168450.084.928914
Produced by FastQC (version 0.11.5)