FastQCFastQC Report
Wed 2 Jan 2019
Ebiom-A_S33_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEbiom-A_S33_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403961
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCCCAGCTTTATCCCCTCAGCAACATCTCCCTGAAGGAGGGTGCTG88912.2009550426897646No Hit
CTTTTTCGTGGTCGGTGTGGCCGAGTCCGTTGGAGTCTGCGTCGTCATCG35950.8899373949465418No Hit
ATCGAAGGGGATCCGCAGTCGTTTGATGATAGCGTCTTCAGCGTTGATGA34880.8634496894502192No Hit
GCTTGGAGTACGCGCCAACCTGATTCAGCACGAGGTTCTCAGCGATGGAG32140.7956213594876733No Hit
GTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTC25250.6250603399833152No Hit
CTCGCCTGGACATCAAGGACCTCACCGTGGCTGACGACCGCGGCTCAGTC20450.5062369882241107No Hit
AGCTCGGCCAGCTTGAAACCCCTGAACGCGGCGATCTTGTCGCGGGTGGT19230.47603605298531293No Hit
ATCACAGGCCTCACCCTCAGGAAGCCCACAGCTCAGCGGGGATGATGGTA18580.4599453907679207No Hit
TGTTAATCTTATTTTGCACTGCCTGGTTGGCCAGGTCCACCACGGATCCC18250.45177628533447534No Hit
GTTTTGCTCCAGCGCGCTTACAAAGTCCCACAGATGCATTAAGATCAATT18050.4468253123445085No Hit
GGGAGCCCCCCAAGGCACAGTCCCCCCGCCCGGCAGTGGCCCCGGGGCTG16550.4096930149197571No Hit
AGCCTTCACCCGGACCGGAAGAATGCACGATCACCGGCACCGAAGGAGAT16430.406722431125777No Hit
TTACGACATGACCTCACGCACCCTGTTCGATCGGCAACCCGCACCAGTCA16300.40350429868229853No Hit
GCCTGGCGGCGAGTGGCGAACGGGTGAGTAATACATCGGAACGTGCCCTG15530.38444305267092616No Hit
GACTCCTATATCTGGAGCTTGGCGTGCGCGGAGCCGCCTTCAGATTCTGG15390.38097737157794936No Hit
AGGGGGGCAAGTCCCGCGAGGAGATGGAAGGAGTTCCTGTCTGGCGGCGG14670.3631538688140687No Hit
CTTGTCTCCGGCCTGATACTCGATGCTGGTGATGTCGCCCTGGAACTCGG13800.34161713630771284No Hit
GAAGCCGGACTGCGGGCGCGCCTCCAGCCAGCCCTGGGCTTCGAGCTTGC13290.32899215518329744No Hit
TGTCTTCGTGCGCCCGGCCGACGGTGTTGATGATGACGCGCCGCTCGGTG13270.3284970578843007No Hit
GGGGATGGCCGCCCGCGAGTACCAGTCGCTGCGGCTGAAGTACATGGGCG13200.32676421733781236No Hit
ACGGCGGCGACACCCTCTGCGGTGCGGCCACGCTGTCGTGCAGCCCGAAC13160.325774022739819No Hit
GCGGCGTCGCGGGTGGCGGAGGTGGCCACCATGCGCACGCGGGTGACGTG12920.3198328551518587No Hit
GCCCTTCCTCCCGGCTCTGCACCTCGGAGGCGACCAGGTTTTCCGCTCCC12760.3158720767598852No Hit
CTGCGAACAACTGGCGGTACGTGGGGATGGTCAAGGGGTGAAGCGGGTTC12220.3025044496869747No Hit
GTCCTGCCCGGTGCAGACCCTCGGCCACGGAGCCCAACTCGCGAGTCGCG12200.30200935238797805No Hit
AAGGCCTTGAAGTCTCCGCTCAGGACATCGACGGCGCCAACGTGCAGCGT12100.29953386589299463No Hit
GACACTTCCCTAAGGAAGCATAATGCAGGAAGGAGGAACTGATGCCCCTT11990.2968108307485129No Hit
AGGCGGTCAGCGTCGATCAGATCCTGGCGGCCCGGCGCGGCGGCCCGGCA11890.2943353442535294No Hit
ATCGCCCGAGGCGAGGGTGGTCGAGCAGGTGGAGCCCGAGCAGGTCCACA11040.27329370904617034No Hit
GCGGAGGTTCGGGCCCAGATCGGGCGGCAGATCCTCGGGGTGCCGGCAGC10960.27131331985018353No Hit
GGCGCCAGACAGGTCGATCAGCGTGCAGCCGGCGGCGGTGGCGCGTGGAG10950.27106577120068526No Hit
CCACCATCACTGCTACTGCTACTATGCCCCAGACTCTCTGTGCTGCTGCC10690.2646295063137283No Hit
TGGGAGCACAGTCTCTGACGCCCATCGCCAGCCCCACGGAGAGGATCAAA10400.25745059547827637No Hit
GTAAAGGTGCATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTC10360.256460400880283No Hit
AGCCGCGGGATACGCGGCATCCGGCGTATGTGCGAGGCTGCGGCGCGTGC10220.2529947197873062No Hit
GCGATGTTCCGCCAGCCCACCCCGGGCTGGCCAGAGTCCACGCCGACCGC10130.2507667819418211No Hit
TTGGCGAACACCTGCTCGTCAATCACGCTCGTTGCCATCTCACGCTCCCT10080.2495290386943294No Hit
CTTGGCGCTTCTGGAGCGGCGTGTTCCTGGGACGGTACCGGACGGCGGCG10070.24928149004483105No Hit
CGGGTCCGGATATCCGACTTGTTGTCGGCCGCCACCCGCTTCAGCCGGCA10000.24754864949834265No Hit
CGAAGCGTGGGGCGGCGGCGAGATCGACTTCCTGGCCGTTTTTGGCCTGC9680.2396270927143957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9590.2373991548689106No Hit
GTCCTCGGTTGGAATAGGCTATTCGCCATGGTCTTTCAGTCCTGATACGG9550.23640896027091723No Hit
TGCCGTTCGAGTTCATGCTCAATGCCTTGCGGCTCAAGGACGGGGTGGCC9380.23220063322944542No Hit
AGCTTTCAGAGTGGCCACGGAGGCAGCCGCAACGATGAGGAGTACTGCGA9340.23121043863145205No Hit
ACCTCGTCGACGACATCACCACCGAGCTCCAACAGGCCTCCTGCGCGGTG9120.2257643683424885No Hit
GCGAGCGAAAGTGGATGATGGCTAAACCATATGCGTGTGAGACTTGCTAG8950.2215560413010167No Hit
TTTCCGAGGTAGTTACCCACGTTGTGGGCAACGACGCCATTGTTCATGGT8930.22106094400202No Hit
CCCGCACCGGGCGCGCCAACCAGCACGGTTCCCGCGACCCCTTCGACCAC8860.2193281034555316No Hit
CGTCAACACGTCCTATGCCGGTTTTGGCCAGGCGAGCTACGAGCTGCTCC8540.21140654667158462No Hit
CTTGAGCTTGCACAGCTCCTCAGCCCGAAGCCCGGTGTGCAGTGCCACCA8430.20868351152710288No Hit
GTCCCCGACACCAGCGTGGAGGAGGCGCGCCAGCTCTGGCAGCAGGGCGT8370.2071982196301128No Hit
ACGGTCCACCGCGTCTCCGCGGGCCTCGAAACTCTCCACCGGACTCTGCG8330.20620802503211944No Hit
CTACCCGTCCTGGACGCAGGAGATCTTGAGGGACATGCTGCAAAGACACG8300.20546537908362442No Hit
CAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCTCGGGCCCC8290.20521783043412606No Hit
GGACGATGTCGGCGTCAAGGATGGCGCAAGACACACGAAGAGGTGGACGA8180.20249479528964429No Hit
ATCATCTGCGCCTGCAAATCGGTAGCGAAGCCGGGGAAAGGCAGGGTGGA8110.2007619547431559No Hit
CTCTGGAGATGCTCCTATCGCCCGCGCGAGTTCTTCGCGGGCGAGGTCCA7950.1968011763511824No Hit
GGCATTCATGGGCACGGTTCAAAAGTATGCGGGCATCGAGGATGGAATCA7820.19358304390770395No Hit
GCGGTGATCGCGGCGGCCAGTGCCGCCCCGGCCATGCGCGGCGACAAGCG7820.19358304390770395No Hit
GGGACCTTGTCCTCGGCGGGCGGCCAGGAGATCAGTTTCCTGGCGAACCC7770.19234530066021224No Hit
AAGAGCCGCTGGTGCGCGAGCAGTTCTCCAAGGCCAAGCTGCGCGGCGTG7720.19110755741272054No Hit
GGATATCCAGGCAGTTATGCTGCAGGCCTTCTTCTGGCAGCGGCAGCAGG7530.186404133072252No Hit
GACGGCCTTGCTCGCCGCTTTCCAGGGCCTGGGCCACATCGGGGGAGACG7430.1839286465772686No Hit
GTAGAGTATACCGAGGCGATCGAGAGAATCATGGTGAAGGAACTCGGCAA7430.1839286465772686No Hit
TGGAAGGTTTCCCCATCAAGGTTGCCTTGATTAACAACGGTAACCTGGGC7380.1826909033297769No Hit
GACCTCTCCGATGCGCTCGCGCAGCTCCTGCATGTCGTCGGCGGAGTACA7360.1821958060307802No Hit
CCGCCGACCAGCCGTACGTGGCGGCGATCTACGTGGAGACCACGCCGGTG7340.1817007087317835No Hit
GGACATCACCGCCCACATCGTCAGCGACGCGCACCACACAGCGATAGCCA7340.1817007087317835No Hit
GCGCCTGTGTAATTGCATATTTGGACGAGATCCCTAAGTCTAAACTCAGA7250.17947277088629843No Hit
GCTGAAGACGGGCGTGGTCCAGACGAATTCGGTCAGGTGCCAATCGAGCT7250.17947277088629843No Hit
AGATCTTGCTGTCCTGGCAGTTGTTGTCCTTGGGGAAGACCGCCAGGCCC7120.17625463844282No Hit
AAAGCCGTCATCCGATGGTGAAAAAAACCTTTATTCGTACCTTTGCTTCG6880.17031347085485973No Hit
GGCTGGAGCGGAGGTCGGCGTGGTGGTCGAGCGCGAGCGCCGCTTCCCAC6800.168333081658873No Hit
GCGGCCGAGGGTGATACCCTGCGGACGGTCAAGCAGCGTGGCGAGCTGAT6740.16684778976188294No Hit
CGGCAAGCAGCCGGCGCTGGTGCTGGCCATGTGCGCCGCCTACCTGGTGT6740.16684778976188294No Hit
GCTCAAGATACTTCTTCCCTCGACCCGAATCAGATCACAGGGGAAGTCGA6720.16635269246288625No Hit
ATGACCACAGCTCCAACGAGCAGCTTGCGGCTGACGACCAAGGTCTGGTG6700.16585759516388957No Hit
GCCGGCATGGCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGATG6670.16511494921539457No Hit
CCACAAGGTCGTCGACTGCCAGTGGGCCTGCCCCGCCCACACCCCGGTTC6640.16437230326689953No Hit
GCGCGCGCTGCTGCAGATGCGCGCGGTCCACGGTGCTGGTGCCCGGCAGG6630.16412475461740117No Hit
GCGAGGCTCAGGGAGTCGCCGCCGGTGTTGCACATCAGATGCGCGAGACT6470.1601639762254277No Hit
GGTGGCGCTGACGAGGATGAACGGGCGCGTCCACAGCGGCGGGCGCGGGG6420.158926232977936No Hit
GGAGCATCCAACACCGAGGCGTAGTACTGAGCCACCAGCAGGATCAGGCC6400.15843113567893932No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT6370.15768848973044428No Hit
GCCAGCGCGCGCTCGGAGCTGCGCTGGATCACCGGACCGTCGGCCATGGG6340.15694584378194923No Hit
GTGAGGCCCATCCCGCCACGCGCCACCGCGACGACGCATCCGACCATGAG6150.15224241944148076No Hit
GCCGGAATAGCACCTTCCAATCCGCCACCGGCTGCGCGAAATCCACGTCC6120.1514997734929857No Hit
GTGACGCTGAGCGGCGATCACACGCTGAGCACCACCACCGGCGGCGTGGC6120.1514997734929857No Hit
GAAACCTACAGCATGATCACGGTCAACGCTGACGATCACGCATTATTCCA6060.15001448159599567No Hit
CTGCCGCTGAAGCTCGTCGAAGTCACGATAAATGGTCATCACGCTGACCC5890.1458061545545238No Hit
CTGACTTCTTTGTTCGTAGGCGGGCTGGAACCACTGAGGGTCGCGCTCGC5880.1455586059050255No Hit
CAGGGCCACGCGTTGGGTGTTTGTGCAGCTCAAGTCCAACAAGACCGCCG5800.14357821670903873No Hit
CGCGTTGAGACGTAGGATTTCTCCTTTAGTCTGGCCTAGAATGTGTCGTA5800.14357821670903873No Hit
GGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAA5780.14308311941004206No Hit
AGACCACGTTCGCCCCGATCGCCGTGCCCGAACGCACTTCCTTGGCCACC5760.14258802211104538No Hit
GTACGATCCTAGAACCAATATCGTGACGATGGGTCGCGGCCCCGAGAAAC5730.14184537616255033No Hit
CGGGAACGTGGGCCCGGGAGGACGGGATGTCGAACGGGTGGATGTGGCGC5680.14060763291505865No Hit
ATAGATTGGTCGCATTCCCTTGCGGCATCGGGGTGGCGCACCAGCTTTCT5680.14060763291505865No Hit
CGCCGTCGATTTCGCCTCGGTCTGCCATCTGGACGCCGCGGCGCAACGGC5670.1403600842655603No Hit
GTATGTGCTGGGCACGGAGCGCCACGAATCGCGGCGCATAGACAACCAGC5670.1403600842655603No Hit
CCTCGACTCGCTCGCGGACATCGTCTCCTTCGGCGTCGTACCGGCGTTTG5630.13936988966756692No Hit
CTCGATCCACCTGCTGAAGACCTTCATCGCCGCCGGCGTGCTCAACGGCC5580.1381321464200752No Hit
AGCAGACCGGCGAAGATGCCGACCACGGCCCCGAGCAGCGGATAGGCCAG5520.13664685452308517No Hit
GGAACTGCTCGGAGCTTCGATAGAGTCCATCCAGAAGGCCGAGGACCGGC5440.1346664653270984No Hit
ATCGAGCACCAGGTTCGCCTCGCCGAGAAGCTGGTGGAGTTGTCGCCGAT5390.13342872207960668No Hit
GGGGGTGCAGATAATCATAATCGTCGCGCTGACGGCGGCGTTTCTGGTCT5360.13268607613111166No Hit
GGCACCCAGCGAGTCGGCGAACTTCTCGATCGGCTCGAAGTTGCCACCCG5270.13045813828562658No Hit
CGAATACTTCGCCTGCATCGTCGCCGTCGCCTCGGCCAAAGCCGCATCCA5210.12897284638863651No Hit
GCCAAAGTACGGGACCAGACTTCTTGTTGCCGTTGGGCCCTGGAAGGGAT5170.12798265179064314No Hit
GCTTTTGGCGGCACACACCACCATTGGCATTGAGCCCAGGCGTTTGGAAA5150.12748755449164648No Hit
GTGCGCCATAGGCGAGGTGCTGGGGATGGTGATCGGAACTTCGATGGGGT5100.12624981124415474No Hit
GTCGACCGTGTCGGTCCACGGTGACTGGCCGTGGTTGGGCAGGTCCACCA5090.1260022625946564No Hit
GACGCCTCGATCTCAGCCTCGTCACGATCGGAAGGCGTCACAGGTCAACT5080.12575471394515805No Hit
CGAACGGCAGCGCGGGCTTCGGCCTGGCGGCGAGTGGCGAACGGGTGAGT5070.12550716529565975No Hit
GAGTTGCCGCCGCCGCCGCCGCAACCGTCTGGGCTGAGATGGTGGAGGTT4910.12154638690368624No Hit
AACCGGCTCTGCGGCTGCGGCGAGCCTTTCAGGGAGTGTCCGGTCTGGAG4890.12105128960468957No Hit
GTCGCCGCCGTGCGCGGTGAGCTGGGGCTGCCGGAAGCGACTCCGGTCTA4870.12055619230569288No Hit
ATTGTGTGCTGTTTCTCTTTTCTCCACCATTCTATAGGAATAAGATGGTA4860.12030864365619454No Hit
CCGGTTTTAATGGTTTGACTGACGAGTTTCATCCGACTTGGGTGTGCGTT4840.11981354635719785No Hit
CGGGATTGTTTGCACTGTCTCGCCATCCTTGCCGAGATGGATACAGTTTT4830.1195659977076995No Hit
CGTCCCGTCGCCTCTACCATCCGCTCCCGCAGCTTCATCCTCGTCCTCCC4820.11931844905820116No Hit
ATGAGCCCGATGCGGATCACCATTGCGGGCGCGGCGATGATGACGATGCT4790.11857580310970614No Hit
GGAGAACTCGGACACATGGTCGTCGAGCCCGAGGGACCACGTTGCAACTG4780.1183282544602078No Hit
GCAGCACCACGCCCACCGTATTGGTGCGGCGGCTGCTCAGGCTGCGGGCG4750.11758560851171276No Hit
CAGCTCCTCTTTGGCCTCCTTGAGGTCCGAGTGCGAGCCGCAGTTGGCCA4720.11684296256321773No Hit
GTCGACCTTTTCATACTTCGCTGCCGGGTCCGGCTGGAAGTGCAGCTCGG4700.11634786526422106No Hit
GTCTGCCTTCCGCAGTCCATTTATTCCAGCCGCCGTCGAGGACGTAGGAC4630.11461502471773265No Hit
GCCACCGTGCCGGCAACCGGCAGCGCGTGCACCGGCAGCGCCACGAGCAT4600.11387237876923763No Hit
TTTCTCTACGCCGTCGACTACTTGCTTGGTGTAGGTGGTGACAGGACGCT4580.11337728147024094No Hit
GGTCATCGTTGGAACGAACAACCTGGGTACCGAACGGGAAGCAGGTGACG4500.1113968922742542No Hit
CCCACCGCGAGATACGGCAGCACCTGAAGCAAGAGGTCGAGGTCGCGCCC4500.1113968922742542No Hit
TGCACGTTCATCATGAAGCTGCCGTCGCCGGAGACCAGCACCACGGTGCG4460.11040669767626082No Hit
CCCGCTGCGGTCGCCGGAGTCGTCGAAGTAATAGACTCGCACATACCCTC4410.10916895442876912No Hit
ACACGCCGAGCTACGCTCCATCGGCGTCCGCTGCGGCAGGAAACGAGTCG4400.10892140577927077No Hit
GGCGATCAGGGCCAGCGGCACCACCGAGCCGCCCGCCACCGACACACCCC4370.10817875983077574No Hit
GGCCACCGAGTCCCACACATCATTGGTGATTTCGTCGCGACGCAGATACC4360.1079312111812774No Hit
ACCGCAGTCAGCGTCGACGGTGAGAATCCTTCGGGAGGAGAGACGTCGCG4330.10718856523278238No Hit
GCAGTACAAAGGCATTGACCTGTGCGGCAAATGAACCAATCACCGTAAGC4310.10669346793378569No Hit
GGACAACGCAGAGCGCACGCTCAAGCCGTCGATGCTGGCCACGCTGATCA4310.10669346793378569No Hit
ACAGGAATGGCCCGCCGCAGCTCACTCAGGCGATGTCCGCACTGCCGCTG4260.10545572468629398No Hit
CACCGAGTGGCGCAGCTGTACAACAGACGTGGCGAGATAGTCCAATCGCT4250.10520817603679564No Hit
CCCGGCGCCACCGCCATCCCGCTCGAGATCCCGCCCTTCACCCTCGTCGG4220.1044655300883006No Hit
GCAGGATTTGGTCTTTATCGATTTGCCCCAATTGCGATATCATGCCATCT4200.10397043278930392No Hit
GGTGCGGCTCGCTCTGCTGACACTCGACGACGAGCCGGAGATGCCCGGCG4190.10372288413980557No Hit
GCGCTGCTCGCCCTCGCCCGGCGCCGCTCGGACGTCCTCTACGCCATGAT4180.10347533549030723No Hit
ATTCAGCAGCTGGTCCGCAAGGGCCGCCACGATAAGTCCACCAAGGTGGC4160.10298023819131055No Hit
AGAGAACCACATTTAAGGAACTCGGCAAAATACCTCCGTAAGTTCGCGAG4130.10223759224281552No Hit
AACTCCCGCGCTCTCTCCTCGGAGCCGAAGACCGGGATCGCGGCGCGCTC4120.10199004359331718No Hit
GCCCACAAGGTCTACTGGCTCACCCAGTCGGGCAACGCCACCGCGCGTCG4090.10124739764482214No Hit
CGCCCCCGAGCACAAGTTCCCGGCGGCGGTCGAGGACTGTCACGCAGCGA4070.10075230034582547No Hit
GGTGCGGCTGGTGGGCCCCGCCACGCTGATGCCCTCGGGCGCCGCCGACT4050.10025720304682878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATT2000.0130.478167
CAGCTTT11700.0123.910888
AGCTTTA11800.0123.505689
CGATTGT550.0118.645889
TTAATCT2200.0118.601823
TCCTATA1850.0117.54794
TACGCTA256.4417036E-6116.0667951
AGTATAC1000.0115.980595
TACGACA2700.0115.4865342
ATCTTAT2400.0114.772466
GTTAATC2450.0109.5121462
TGGAGTA5100.0109.442474
GGGATCC4550.0108.333518
CGTCCTA1150.0107.1825269
AGTACGC5200.0105.94387
TGTTAAT2550.0105.256661
AGACGTA900.0104.7047048
TATCACG352.7614988E-7103.554115
CATACGT352.763536E-7103.54128145
TTACGAC2950.0103.279781
Produced by FastQC (version 0.11.5)