FastQCFastQC Report
Wed 2 Jan 2019
E1961-R_S28_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE1961-R_S28_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75830613
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG964730.12722170662130872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG118250.027.3422769
GCCGGGC209900.025.8237381
GCTAAGT105950.024.7049482
AGAGCGG275150.022.844896145
CGGGAGT193600.022.5632721
CGCTAAG101850.022.37071
TGTACTA145800.021.8260868
GCGACAT134100.021.6442321
GCGGAAT260300.021.186071
GCGGGAC296850.020.6552581
CGGAATC261450.019.5791472
TTCTCGT126750.019.043746
CCGGCCT210300.018.7702731
CGGCCTA84250.018.5031742
GGCTAGC594800.018.0606446
GCCGACC593000.017.5103931
CGGGTAT114550.017.1666361
GCGGGCT263850.017.1333351
CCGACCC527000.017.101662
GCGGGTT521250.017.0530091
Produced by FastQC (version 0.11.5)