FastQCFastQC Report
Wed 2 Jan 2019
E1961-R_S28_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE1961-R_S28_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75830613
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGC182950.028.774581
TCTCGTG139950.028.7513037
GCGGAAT282300.026.2252521
CGGAATC275250.025.0785472
GCGACAT154650.022.7872091
TTCTCGT129350.022.5909966
TTAGCGC70150.022.1198846
GTACTAG126300.021.2964089
TGTACTA129900.019.3109078
TACGCCC187000.018.3769847
CCCTTCG173000.017.7711935
GTCGGAT280550.017.627021
GGGAAAT396100.017.5923291
GGACTTA132250.017.4355741
TCGCTAT130750.017.2446528
CGATTCA123550.017.137074
TACGCTC112250.016.8595872
GTTACCG133550.016.72291
TCTCCGG241750.016.70656
GCGGGAC206550.015.9029541
Produced by FastQC (version 0.11.5)