FastQCFastQC Report
Wed 2 Jan 2019
E1961-A_S29_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE1961-A_S29_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92033920
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1918940.20850356042641668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG76850.044.4298489
CGAACGT288150.038.8153343
TAACGAC308650.036.2877469
TCGATCG104100.033.6319777
GTAACGA347550.032.1611378
CCTATTC117100.030.897132
GTCGGCT139350.030.4108371
GCGGAAT304700.029.8639741
AACGTAA384250.029.0319085
GCGGACT279750.028.455031
CGTAACG393200.028.2976867
CGTTCGG70550.026.9190254
TCTAACG117800.026.8900857
GTCGGAG103100.026.8860631
GGCGAAC470300.026.646481
CGGTTCA329100.025.6227862
CGGGTAC123950.025.5583449
CGGAAAG129400.025.4387662
ACGTAAC515900.024.939286
CGACTCT116750.024.8344145
Produced by FastQC (version 0.11.5)