FastQCFastQC Report
Wed 2 Jan 2019
E1961-A_S29_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameE1961-A_S29_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92033920
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA92300.030.7121268
TACTTCG87850.029.136014
TAGGTCG94200.028.707998145
CCCGGCG135400.024.0950978
GTAGCTA195800.023.9971396
GGCGATC298250.023.7774981
GCGGAAT296850.023.5965121
TTACCGC172400.023.592316145
CTTCGCG128950.023.3920946
GCGATAC128500.023.3617271
TATTCCG79050.023.2985534
CGAACGT187050.023.1039183
ACCTCTG395650.022.8534836
TAACGAC190750.022.4244639
CGGGTAC132300.021.8101069
CGGAATC290650.021.4546412
TTCGTCT138950.021.2911325
TCTAGCG76050.021.2589197
GTTCGTC130650.021.256761
CGTCCAG226100.021.163087
Produced by FastQC (version 0.11.5)