MinION Mk1B (MN39381) Final report

— · 07-11-23_test-metaG-vp2pd2-anses · no_sample · MN39381
Protocol run ID: 801271ca-28df-4f0b-b976-929fd04e4631

DATA OUTPUT

Estimated bases
9.65 Gb
Reads generated
27.69 M
Estimated N50
356
Total data produced (pass / fail)
117 GB

RUN DURATION

Run time

44 hrs 54 mins / 72 hrs 0 mins (est)

Elapsed time

Run limit

Run status

Unknown

View unit abbreviations used in this report
Flow cell type
FLO-MIN114
Flow cell type alias
FLO-MIN114
Flow cell ID
FAU98710
Kit type
SQK-NBD114-24
Run limit
72 hrs
Active channel selection
On
Pore scan freq.
1.5 hrs
Reserved pores
On
Minimum read length
20 bp
Basecalling
Off
Modified basecalling
Off
FAST5 output
Off
FASTQ output
Off
BAM output
Off
Bulk file output
Off
Data location
MinKNOW
23.07.12
Bream
7.7.6
Configuration
5.7.11
Guppy
7.1.4
MinKNOW Core
5.7.5

The read length graph shows the total number of bases vs the read length. The longest 1% of strands are classified as outliers, and excluded to allow focus on the main body of data.

Legend

Basecalled
Estimated

The longest 1% of strands are classified as outliers, and aggregated into groups to show their relative amounts.

Read length (Mb) Aggregated reads (Mb)
0.453125 - 0.703125 44.13
0.703125 - 0.953125 24.39
0.953125 - 1.20312 14.12
1.20312 - 1.45312 12.13
1.45312 - 1.57812 1.64

The cumulative output shows the total amount of bases or reads sequenced over time by your device.

Bases

Legend

Estimated
Predicted total number of bases, prior to basecalling

Reads

Legend

Total
Total number of reads, including passed, failed and skipped.

The Pore activity graph shows the performance of your sample as it is being sequenced during a run.

Legend

Sequencing
Pore currently sequencing
Adapter
Pore currently sequencing adapter
Pore available
Pore available for sequencing
Unavailable
Pore unavailable for sequencing
Active feedback
Channel ejecting analyte
No pore
No pore detected in channel
Out of range-high
Current is positive but unavailable for sequencing
Out of range-low
Current is negative but unavailable for sequencing
Multiple
Multiple pores detected. Unavailable for sequencing.
Saturated
The channel has switched off as current levels exceed hardware limitations
Zero
Pore currently unavailable for sequencing
Channel disabled
Channel is disabled and awaiting another pore scan
Unclassified
Pore status unknown
Sequencing
Pore currently sequencing
Pore available
Pore available for sequencing
Unavailable
Pore currently unavailable for sequencing
Inactive
Pore no longer suitable for further sequencing
Unclassified
Pore status unknown

General

Some commonly seen issues are excess pores classified as Recovering, Open Pore, or Free Adapter. To find out what advice is applicable for your run, visit the user guide.

A Pore scan is performed at configurable time intervals to determine the current status of pores within channels on a Flow Cell. For this run a Pore scan is performed every 1.5 hrs.

Legend

Pore available
Pore in channel available for sequencing
Reserved pore
Pore in reserve, will return to available when required
Unavailable
Pore inhibited from sequencing
Saturated
Possible contamination in the sample
Zero
No current is passing through this pore, possibly due to bubbles on the membrane
Inactive
Pore no longer suitable for further sequencing

High proportion Unavailable

Possible contaminants in library blocking the pore. Consider using the Flow Cell Wash Kit, and reloading a library. 

High proportion Inactive

If localised to one area of the Flow Cell, this could indicate that an air bubble has been introduced during the flushing/loading steps. If inactivity is spread across the Flow Cell this could be caused by improper loading of the library, please refer to the user guide for further support.

The temperature of the Flow Cell over the run time.

Legend

Measured
Target

Out of range

Check that the Flow Cell is correctly seated and firmly pushed down into the device. Ensure ambient temperature is always within the specified range for your device in the user guide.

Air flow should be good but not excessive. Excessive amounts of cool air blowing on the device could prevent it from reaching target temperature.

SYSTEM MESSAGES

System messages are a record of the events that occurred in the time covered by this report.

Error · 09 Nov 23, 11:58
MinKNOW failed to communicate with the device. Please unplug and re-plug your MinION, or turn your GridION/PromethION off and on again, and restart the experiment.
Error · 09 Nov 23, 11:58
MinION disconnected during experiment. Please click Stop, and unplug and re-plug the MinION, then start a new experiment.
Error · 09 Nov 23, 11:58
There was an error receiving data from the device. Try re-plugging the device if possible, or rebooting the system. If the issue persists, please contact Support
None
Disk space · 07 Nov 23, 14:59
Disk / has 1430 GB space remaining
Waiting for temperature · 07 Nov 23, 14:59
Waiting up to 300 seconds for temperature to stabilise at 35.0°C
Starting · 07 Nov 23, 15:04
Starting sequencing procedure
Pore scan starting · 07 Nov 23, 15:04
Performing Pore Scan
Pore scan result · 07 Nov 23, 15:06
Pore scan for flow cell FAU98710 has found a total of 1488 pores. 507 pores available for immediate sequencing
Pore scan starting · 07 Nov 23, 16:37
Performing Pore Scan
Pore scan result · 07 Nov 23, 16:40
Pore scan for flow cell FAU98710 has found a total of 1442 pores. 507 pores available for immediate sequencing
Pore scan starting · 07 Nov 23, 18:11
Performing Pore Scan
Pore scan result · 07 Nov 23, 18:13
Pore scan for flow cell FAU98710 has found a total of 1391 pores. 497 pores available for immediate sequencing
Pore scan starting · 07 Nov 23, 19:44
Performing Pore Scan
Pore scan result · 07 Nov 23, 19:47
Pore scan for flow cell FAU98710 has found a total of 1293 pores. 479 pores available for immediate sequencing
Pore scan starting · 07 Nov 23, 21:18
Performing Pore Scan
Pore scan result · 07 Nov 23, 21:21
Pore scan for flow cell FAU98710 has found a total of 1212 pores. 464 pores available for immediate sequencing
Pore scan starting · 07 Nov 23, 22:52
Performing Pore Scan
Pore scan result · 07 Nov 23, 22:54
Pore scan for flow cell FAU98710 has found a total of 1168 pores. 457 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 00:25
Performing Pore Scan
Pore scan result · 08 Nov 23, 00:28
Pore scan for flow cell FAU98710 has found a total of 1118 pores. 435 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 01:59
Performing Pore Scan
Pore scan result · 08 Nov 23, 02:01
Pore scan for flow cell FAU98710 has found a total of 1059 pores. 416 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 03:32
Performing Pore Scan
Pore scan result · 08 Nov 23, 03:35
Pore scan for flow cell FAU98710 has found a total of 1012 pores. 418 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 05:06
Performing Pore Scan
Pore scan result · 08 Nov 23, 05:08
Pore scan for flow cell FAU98710 has found a total of 961 pores. 392 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 06:39
Performing Pore Scan
Pore scan result · 08 Nov 23, 06:42
Pore scan for flow cell FAU98710 has found a total of 897 pores. 360 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 08:12
Performing Pore Scan
Pore scan result · 08 Nov 23, 08:14
Pore scan for flow cell FAU98710 has found a total of 866 pores. 382 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 09:45
Performing Pore Scan
Pore scan result · 08 Nov 23, 09:48
Pore scan for flow cell FAU98710 has found a total of 811 pores. 357 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 11:18
Performing Pore Scan
Pore scan result · 08 Nov 23, 11:21
Pore scan for flow cell FAU98710 has found a total of 766 pores. 328 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 12:51
Performing Pore Scan
Pore scan result · 08 Nov 23, 12:54
Pore scan for flow cell FAU98710 has found a total of 722 pores. 342 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 14:25
Performing Pore Scan
Pore scan result · 08 Nov 23, 14:28
Pore scan for flow cell FAU98710 has found a total of 675 pores. 311 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 15:58
Performing Pore Scan
Pore scan result · 08 Nov 23, 16:01
Pore scan for flow cell FAU98710 has found a total of 632 pores. 305 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 17:32
Performing Pore Scan
Pore scan result · 08 Nov 23, 17:34
Pore scan for flow cell FAU98710 has found a total of 595 pores. 291 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 19:04
Performing Pore Scan
Pore scan result · 08 Nov 23, 19:07
Pore scan for flow cell FAU98710 has found a total of 554 pores. 284 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 20:37
Performing Pore Scan
Pore scan result · 08 Nov 23, 20:40
Pore scan for flow cell FAU98710 has found a total of 503 pores. 288 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 22:10
Performing Pore Scan
Pore scan result · 08 Nov 23, 22:12
Pore scan for flow cell FAU98710 has found a total of 441 pores. 240 pores available for immediate sequencing
Pore scan starting · 08 Nov 23, 23:43
Performing Pore Scan
Pore scan result · 08 Nov 23, 23:45
Pore scan for flow cell FAU98710 has found a total of 409 pores. 235 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 01:15
Performing Pore Scan
Pore scan result · 09 Nov 23, 01:18
Pore scan for flow cell FAU98710 has found a total of 401 pores. 211 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 02:49
Performing Pore Scan
Pore scan result · 09 Nov 23, 02:51
Pore scan for flow cell FAU98710 has found a total of 397 pores. 225 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 04:21
Performing Pore Scan
Pore scan result · 09 Nov 23, 04:24
Pore scan for flow cell FAU98710 has found a total of 362 pores. 201 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 05:54
Performing Pore Scan
Pore scan result · 09 Nov 23, 05:57
Pore scan for flow cell FAU98710 has found a total of 328 pores. 185 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 07:27
Performing Pore Scan
Pore scan result · 09 Nov 23, 07:29
Pore scan for flow cell FAU98710 has found a total of 319 pores. 152 pores available for immediate sequencing
Pore scan starting · 09 Nov 23, 08:59
Performing Pore Scan
Pore scan result · 09 Nov 23, 09:02
Pore scan for flow cell FAU98710 has found a total of 291 pores. 185 pores available for immediate sequencing
Message · 09 Nov 23, 11:59
Sequencing device with flow cell ready
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